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Soaking potato tuber pieces for 15 min in 8,000 mug/ml of oxamyl just before planting reduced the number of Globodera rostochiensis cysts that developed on potato roots, but this treatment was phytotoxic. Five foliar applications of 1.12 kg a.i./ha of oxamyl or carbofuran at 10-day intervals beginning when 90% of the plants had emerged suppressed increase in G. rostochiensis densities. Similar ...

Globodera millefolii and G. artemisiae are interesting because their type localities (Estonia and Russia, respectively) are geographically distant from those of the potato cyst nematodes and other Globodera species that seem to have originated in the Western world, and because the type host for each is a member of Compositae rather than Solanaceae. Sequence data for ITS1, ITS2, and 5.8S ribosom...

The fecundity of Globodera rostochiensis (RA) females that developed on resistant Rosa and susceptible Katahdin potato cultivars were compared. Cysts collected from each cultivar were bulked, separated into four sizes (> 500 mum, 355-500 mum, 250-355 mum, and < 250 mum), and crushed to determine fecundity as measured by viable egg content (VEC). Fewer and generally smaller cysts developed on Ro...

Evolutionary relationships based on ribosomal DNA (rDNA) sequence data for a previously unknown species of Globodera from Portugal, Punctodera chalcoensis from Mexico, and P. punctata from Estonia, plus previously published sequences, support the following relationships: (((Cactodera weissi, G. artemisiae, C. milleri), ((G. sp. Bouro, G. sp. Canha, G. sp. Ladoeiro), ((G. pallida, G. rostochiens...

Linalool is either a toxic compound to a few species of plant parasitic nematodes or attractive to entomopathogenic nematodes. This compound is produced and emitted by several host plants of Globodera rostochiensis and G. pallida, the potato cyst nematodes (PCN). With the aim to reveal the effect of linalool on PCN, laboratory assays were carried out. Survival of PCN second-stage juveniles (J2s...

Potato cultivars Katahdin (susceptible) and Rosa (resistant) were exposed to infective second-stage juveniles (J2) of Globodera rostochiensis for varying periods of time, after which root systems were washed and plants were placed in Hoagland's solution to assess J2 egression and male emergence. After transfer to liquid culture, many J2 egressed from both cultivars, but significantly more egres...

The tobacco cyst nematode (Globodera tabacum solanacearum) continues to pose a serious threat to flue-cured tobacco production in Virginia and nearby states. Soils were sampled from five uninfested and two infested flue-cured tobacco-producing locations. Twenty-three edaphic factors were characterized to determine if any were correlated with G. t. solanacearum reproduction. Comparisons were als...

Although several attempts have been made to differentiate nematode species with polyclonal antisera, these efforts thus far have met with limited success because of extensive crossreactivities of the sera. Since the hybridoma technique offers the opportunity to develop more specific serological reagents, some research groups have recently started to apply this technology to the problem of speci...

Ribosomal DNA (rDNA) sequence data were compared for five species of Globodera, including G. rostochiensis, G. pallida, G. virginiae, and two undescribed Globodera isolates from Mexico collected from weed species and maintained on Solanum dulcamara. The rDNA comparisons included both internal transcribed spacers (ITS1 and ITS2), the 5.8S rRNA gene, and small portions of the 3' end of the 18S ge...