the purpose of this study was to evaluate the use of glass capillary micropipette (gcm) as a vessel forvitrification of bovine oocytes. cumulus-oocyte complexes (cocs) were obtained from slaughter-house andwashed 5 to 6 times in the washing medium (tcm-199 + 20% fbs) and randomly assigned to treatment andcontrol group. in the first step of vitrification, cocs were exposed to first vitrification...

The purpose of this study was to evaluate the use of glass capillary micropipette (GCM) as a vessel forvitrification of bovine oocytes. Cumulus-oocyte complexes (COCs) were obtained from slaughter-house andwashed 5 to 6 times in the washing medium (TCM-199 + 20% FBS) and randomly assigned to treatment andcontrol group. In the first step of vitrification, COCs were exposed to first vitrification...

417 It is commonly, but erroneously, supposed that sharp glass micropipette electrodes were co-invented by Gilbert Ning Ling and Ralph Waldo Gerard (Ling and Gerard, 1949; much as the invention of the ubiquitous “Pasteur pipette” is incorrectly attributed to Louis Pasteur). Actually, fine, sharp-tipped examples of capillary glass microelectrodes had been developed and used successfully from the...

We present a rapidly fabricated micro-capillary electrophoresis chip that utilizes surface-tension-driven flow for sample injection and extraction of DNA. Surface-tension-driven flow (i.e. passive pumping) [G. M. Walker et al., Lab. Chip. 2002, 2, 131-134] injects a fixed volume of sample that can be predicted mathematically. Passive pumping eliminates the need for tubing, valves, syringe pumps...

Ion transfer across the polarized interface between a room-temperature ionic liquid (RTIL) or room-temperature molten salt, tetrahexylammonium bis(trifluoromethylsulfonyl)imide (THAC(1)C(1)N), and water has been studied voltammetrically using a micro liquid-liquid interface formed at the orifice of a glass capillary micropipette. A small current of nanoampere level circumvents the problem of th...

Microiontophoresis entails passage of current through a micropipette tip to deliver a solute at a designated site within an experimental preparation. Microiontophoresis can simulate synaptic transmission by delivering neurotransmitters and neuropeptides onto neurons reproducibly. Negligible volume (fluid) displacement avoids mechanical disturbance to the experimental preparation. Adapting these...

The microinjection of synthetic molecules, proteins, and nucleic acids into the cytosol of living cells is a powerful technique in cell biology. However, the insertion of a glass micropipette into the cell is a potentially damaging event, which presents significant problems, especially for small mammalian cells (spherical diameter = 2-15 micron), especially if they are only loosely adherent. Th...

Glass micropipettes are widely used to record neural activity from single neurons or clusters of neurons extracellularly in live animals. However, to date, there has been no comprehensive study of noise in extracellular recordings with glass micropipettes. The purpose of this work was to assess various noise sources that affect extracellular recordings and to create model systems in which novel...

This paper presents a computer vision-based method for visually detecting the contact between an end-effector and a target surface under an optical microscope during microrobotic manipulation. Without using proximity or force/touch sensors, this method provides a submicrometer detection accuracy and possesses robustness. Fundamentally, after the establishment of contact in the world frame, furt...