A technique combining PCR and restriction fragment length polymorphism analysis was used to generate specific DNA fragment patterns from spore extracts of arbuscular mycorrhizal fungi. With the universal primers ITS1 and ITS4, DNA fragments were amplified from species of Scutellospora and Gigaspora that were approximately 500 bp long. The apparent lengths of the corresponding fragments from Glo...

The present study deals with the diversity and distribution of VAMF at different sites with different selected plants. Maximum number of VAMF species were found at site IV (57 species) out of which Glomus species was most dominant (58%), followed by Acaulospora (19%), Scutellospora (8%), Sclerocystis (4.8%) and Gigaspora (1.6%) respectively. In site II 56 species of VAMF were observed with Glom...

Cassava is widely grown in Inceptisol. As agricultural land, the soil has several problems, i.e., high acidity, easily leached surface layers, unstable aggregates, and slow permeability. Arbuscular mycorrhizal fungi (AMF) form a symbiotic relationship with over 80% of higher plant species. However, effectiveness AMF on cassava not been reported. This study aimed to determine effect inoculants v...

Despite the importance of arbuscular mycorrhizal fungi in the majority of terrestrial ecosystems, their ecology, genetics, and evolution are poorly understood, partly due to difficulties associated with detecting and identifying species. We explored the inter- and intraspecies variations of the 18S rRNA genes of the genus Gigaspora to assess the use of this marker for the discrimination of Giga...

The effect of flavonoids isolated from arbuscular mycorrhizal (AM) colonized and noncolonized clover roots on the number of entry points and percentage of root colonization of tomato (Lycopersicum esculentum L.) by Gigaspora rosea, Gi margarita, Glomus mosseae and G. intrarradices symbionts was determined. With fungi of both genera, a correlation between the number of entry points and the perce...

Using dual cultures of arbuscular mycorrhizal (AM) fungi and Medicago truncatula separated by a physical barrier, we demonstrate that hyphae from germinating spores produce a diffusible factor that is perceived by roots in the absence of direct physical contact. This AM factor elicits expression of the Nod factor-inducible gene MtENOD11, visualized using a pMtENOD11-gusA reporter. Transgene ind...

Gaeumannomyces graminis var. tritici causes take-all disease, the most important root disease of cereal plants. Cereal plants are able to form a symbiotic association with soil-borne arbuscular mycorrhizal fungi which can provide bioprotection against soil-borne fungal pathogens. However, the bioprotective effect of arbuscular mycorrhizal fungi against soil-borne fungal pathogens might vary. In...

Intracellular bacteria have been found previously in one isolate of the arbuscular mycorrhizal (AM) fungus Gigaspora margarita BEG 34. In this study, we extended our investigation to 11 fungal isolates obtained from different geographic areas and belonging to six different species of the family Gigasporaceae. With the exception of Gigaspora rosea, isolates of all of the AM species harbored bact...

The flavonoids 5,6,7,8,9-hydroxy chalcone, 3,7-hydroxy-4?methoxy flavone, 5,6,7,8-hydroxy-4?-methoxy flavone and 3,5,6,7,4?-hydroxy flavone can be detected only in non-mycorrhizal roots of white clover, but not in mycorrhizal roots, whereas the flavonoids acacetin, quercetin and rhamnetin are only present in mycorrhizal roots. We tested the effect of several concentrations of these compounds on...