background and objectives : in iran, anaplasmosis is normally diagnosed with traditional giemsa staining method. this is not applicable for identification of the carrier animals. the aim of this study was to compare the detection of anaplasma marginale in two different numbers of microscopic fields (50 and 100) using conventional giemsa staining method compared with the pcr-rflp technique. mate...

Analysis of micronuclei (MN) in preimplantation embryos is a good method for the evaluation of cytogenetic damage induced by occupational and environmental mutagen during early pregnancy. To examine whether conventional Giemsa staining produced the same accuracy of micronuclei as the DNA-specific 4', 6'-diamidino-2-phenylindole (DAPI) staining in preimplantation embryo induced by maternal expos...

This study was undertaken to evaluate the compatibility of Giemsa staining protocol with the comet assay. We showed, for the first time, that DNA comets can be visualized and analyzed using Giemsa staining. We generated DNA damage dose response curves for human peripheral blood lymphocytes exposed to X-ray radiation using the comet assay with either SybrGreen I or Giemsa stain. The dose respons...

Toluidine blue staining was used to detect Chlamydia trachomatis inclusions in both McCoy and HeLa-229 cells from clinical specimens. This method was more sensitive than iodine staining for detecting C trachomatis inclusions in both McCoy and HeLa-229 cells and also more sensitive than Giemsa staining for detecting chlamydial inclusions in HeLa-229 cells. While its sensitivity for detection of ...

In a clinical study of 190 men with non-gonococcal urethritis, Chlamydia trachomatis inclusions were sought in cycloheximide-treated McCoy cells by an indirect immunofluorescent antibody technique. The method was consistently reliable over a period of two years, and the results were obtained within 24 hours of a patient's attendance. The results correlated with those obtained by Giemsa staining...

Helicobacter pylori (H. pylori) remains a prevalent chronic infection in the developing countries. H.pylori is an important agent associated with peptic ulcer disease, chronic gastritis and gastric malignancies. It can be detected by various invasive (rapid urease test and histology) and non-invasive tests (stool antigen test, urea breath test and serology). The aim of this study was to compare...