the changes of total calcium content of persian sturgeon, acipenser persicus follicles were investigated during different stages of germinal vesicle migration. total calcium content increased during maturation and ripening of the oocytes i.e. migration of nucleus toward the animal pole. according to data, the total calcium of follicles with polarization index (pi: the ratio of the distance of t...

objective: mitochondria is a critical organelle within the cell and has important role in the oocyte development, thus the aim of this study was the comparison of mitochondrial distribution within the mouse oocyte at germinal vesicle (gv), germinal vesicle breakdown (gvbd) and metaphase ii (mii) stages using laser confocal microscopy (lscm). materials and methods: after superovulation of adult...

background: cryopreservation and in vitro maturation (ivm) of oocyte is becoming an important technique in infertility treatment and fertility preservation. also it has been proposed to establish a genetic resource bank for endangered or commercially important animal species. objective: the aim of this study was to evaluate viability, maturation and fertilization rate of mouse immature oocytes ...

in order to determine the most effective hormones on the in vitro oocyte maturation (germinal vesicle breakdown - gvbd) in a hybrid sturgeon bester, ovarian follicles were incubated at the migratory nucleus stage in the presence of several steroids, gonadotropin preparations and forskolin. occurrence of gvbd and 17,20β-dihydroxy-4-pregnen-3 -one (dhp) production were followed during an expe...

background: routine oocytes cryopreservation remained an elusive technique in the wide ranges of available assisted reproductive technologies. the microtubules of oocytes are vulnerable to cryoprotectants and thermal change during cryopreservation. objective: the effects of a vitrification protocol were investigated on the spindle and chromosome configurations of mice oocytes cryopreserved at t...

in the present study, a total of 595 specimens collected monthly in the northern part of persian gulf between november 2006 and october 2007 were examined by routine macroscopic and histological techniques. monthly changes in the gonadosomatic index showed the spawning season extended within 2 peaks, from april- may and september. the maturity stages are morphologically separated according to t...

Phospholipase D2 (PLD2) is involved in cytoskeletal reorganization, cell migration, cell cycle progression, transcriptional control and vesicle trafficking. There is no evidence about PLD2 function in oocytes during meiosis. Herein, we analyzed PLD2 expression and its relationship with spindle formation and positioning in mouse oocyte meiosis. High protein level of PLD2 was revealed in oocytes ...

objectives: it was aimed to investigate the effects of different doses of follicle stimulating hormone (fsh) and activin a on the growth and maturation of preantral mouse follicles during the in vitro culture. methods: preantral follicles (90-100 µm in diameter) were harvested from 6-8 week-old syrian mice and cultured in tcm199 culture medium for 6 days to see the effect of fsh and activin a. ...

Polychlorinated biphenyls (PCBs) are endocrine-disrupting chemicals that mimic estrogen in fish. Among the various PCBs, 3,3’,4,4’,5-pentachlorobiphenyl (PCB126), considered an antagonist, has been reported to elicit estrogenic activity We investigated potency of PCB126 vitro oocyte maturation dusky tripletooth goby, Tridentiger obscurus . In this study, we quantified steroid metabolites follow...

Background: Cryopreservation of oocytes, which is an interesting procedure to conserve female gametes, is an essential part of reproductive biotechnology. The objective of the present study was to investigate the effects of cumulus cells on survival,in vitro maturation,fertilization and subsequent developmental capacity of mouse germinal vesicle stage oocytes during vitrification. Materials and...