UNLABELLED A number of technologies, including CRISPR/Cas, transcription activator-like effector nucleases and zinc-finger nucleases, allow the user to target a chosen locus for genome editing or regulatory interference. Specificity, however, is a major problem, and the targeted locus must be chosen with care to avoid inadvertently affecting other loci ('off-targets') in the genome. To address ...

Genome scans have become a common approach to identify genomic signatures of natural selection and reproductive isolation, as well as the genomic bases of ecologically relevant phenotypes, based on patterns of polymorphism and differentiation among populations or species. Here, we review the results of studies taking genome scan approaches in plants, consider the patterns of genomic differentia...

Our aim is to identify an extraction method and the source of mouse tissue(s) that could allow a high-resolution genomic scan from a living mouse. We compared and optimized two methods for yield, purity of DNA, and their use in the polymerase chain reaction (PCR) of DNA extracted from different mouse tissues. In addition to whole blood, tissue samples from the brain, liver, testis, and tail wer...

The scan of a genomic database aims to detect similarities between dna or protein sequences. This is a time-consuming operation, especially when weak similarities are searched. Speeding up the scan can be managed using various strategies of paralleliza-tion. This paper presents two approaches carried on at irisa: systolic and distributed parallelization.

The scan of a genomic database aims to detect similarities between dna or protein sequences. This is a time-consuming operation, especially when weak similarities are searched. Speeding up the scan can be managed using various strategies of paralleliza-tion. This paper presents two approaches carried on at irisa: systolic and distributed parallelization.