background there are hoarding documents for the biological importance of cyclooxygenase-2 (cox-2) in pancreatic carcinogenesis. we aimed to thoroughly investigate the dna sequence variations of whole cox-2 exons in a large case-control study of pancreatic cancer by direct sequencing.   methods the entire exonic regions of cox-2 including 10 exons were sequenced in the germline dna of 96 patient...

The nature and pace of genome mutation is largely unknown. Standard methods to investigate DNA-mutation rely on arraying or sequencing DNA from a population of cells, hence the genetic composition of individual cells is lost and de novo mutation in cell(s) is concealed within the bulk signal. We developed methods based on (SNP-) arraying and next-generation sequencing of single-cell whole-genom...

objective: type 2 diabetes (t2d) as a complex disease is the result of genetically heterogeneous factors and environmental issues interaction. linkage and small-scale candidate gene studies were successful in identification of genetic susceptibilities of monogenic form of diseases. however, they were largely unsuccessful while applying to the more common forms of disease. by designing genome wi...

in this research, samples were collected from three iranian native dogs and three wolves. whole-genome sequencing for each individual was performed using next-generation sequencing technology. all short reads were aligned to the reference genome using bwa tool. single-nucleotide polymorphisms (snps) and small insertions and deletions (indels) were detected using the genome analysis toolkit (gat...

Recently, we demonstrated chromosome instability (CIN) in human cleavage stage embryogenesis following in vitro fertilization (IVF). CIN not necessarily undermines normal human development (i.e. when remaining normal diploid blastomeres develop the embryo proper), however it can spark a spectrum of conditions, including loss of conception, genetic disease and genetic variation development. To s...

RNA-sequencing is a new method of transcriptome characterization of organisms. Based on identity and relatedness, there are large genetic variations among different cattle breeds. The goal of the current study was to sequence the transcriptome of Guilan native cow and compare with available reference genome using RNA-sequencing method. Blood samples were collected from 14 Guilan native cows and...

In order to perform breeding programs and improve production of native chickens, preserving genetic diversity in different areas of Iran is important due to the reduced available population. Genome sequencing is considered the most functional approach to determine the phylogeny relation between close populations. The aim of the present study was the evaluation of the phylogeny and genetic nucle...