mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. several recombinant protein systems have been introduced to the market, most of which are under clinical development. in spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and pr...

objective: the greatest challenge in cancer gene therapy is to achieve the high specificity and efficiency in targeting of cancer cells. because the goal of cancer gene therapy is to eradicate cancer cells, many therapeutic genes could be detrimental if unintentionally expressed in normal cells. using promoter of the genes which are expressed specifically in cancer cells or have much more expre...

objective(s): p-glycoprotein (p-gp) is an efflux protein, the overexpression of which has been associated with multidrug resistance in various cancers. although sirna delivery to reverse p-gp expression may be promising for sensitizing of tumor cells to cytotoxic drugs, the therapeutic use of sirna requires effective carriers that can deliver sirna intracellularly with minimal toxicity on targe...

abstractgene therapy is a therapeutic approach to deliver genetic material into cells to alter their function in entire organism. one promising form of gene delivery system (dds) is liposomes. the success of liposome-mediated gene delivery is a multifactorial issue and well-designed liposomal systems might lead to optimized gene transfection particularly in vivo. liposomal gene delivery systems...

streptococcus agalactiae is acommensalorganism, but it may cause infection in susceptible hosts. the aim of this study was to evaluate pcr assay compared with conventional culture method for direct detection of streptococcus agalactiae . total of 203 paired low vaginal swabs were collected from women at 35-37 weeks of pregnancy from june 2013 through february 2014 for detection of streptococcus...

conclusions our findings revealed that the zfn technology could be employed to overcome ampicillin resistance by the targeted disruption of the ampicillin resistance gene, which leads to inactivation of β-lactam synthesis. therefore, zfn technology could be engaged to decrease the antibiotic resistance issue with the construction of a zfn archive against different args. to tackle the resistance...

objective: lentivirus-derived vectors are among the most promising viral vectors for gene therapy which is currently available, but their use in clinical practice is limited due to associated risk of insertional mutagenesis. gene targeting is an ideal method for gene therapy, but it has low efficiency in comparison to viral vector methods. in this study, we are going to design and construct an ...

background: human granulocyte colony-stimulating factor (hg-csf) can serve as valuable biopharmaceutical for research and treatment of the human blood cancer. transplastomic plants have been emerged as a new and high potential candidate for production of recombinant biopharmaceutical proteins in comparison with transgenic plants due to extremely high level expression, biosafety and many other a...

Background: Radiation-induced molecular changes on the endothelial surface of brain arteriovenous malformations (AVM) may be used as markers for specific vascular targeting agents. In this study, we examined the level of expression of platelet endothelial cell adhesion molecule-1 (PECAM-1) on brain endothelial cell surface after radiation treatment, with the aim of targeting the radiation-induc...

background: radiation-induced molecular changes on the endothelial surface of brain arteriovenous malformations (avm) may be used as markers for specific vascular targeting agents. in this study, we examined the level of expression of platelet endothelial cell adhesion molecule-1 (pecam-1) on brain endothelial cell surface after radiation treatment, with the aim of targeting the radiation-induc...