Designing and producing a proper fusion construction is the most important problem of producing large quantities of a properly folded functional protein. This construction should have all necessary components of a real gene. A good designed fusion gene construction could be cloned into a good and suitable host. Clostridium perfringens is an important pathogen of humans and livestock and produce...

designing and producing a proper fusion construction is the most important problem of producing large quantities of a properly folded functional protein. this construction should have all necessary components of a real gene. a good designed fusion gene construction could be cloned into a good and suitable host. clostridium perfringens is an important pathogen of humans and livestock and produce...

Fusion protein technology represents the strategy to achieve rapid, efficient, and cost-effective proteinexpression. Epsilon and Beta toxins are the most potent Clostridial toxins and cause disease in animals.This study describes in silico fusion of Clostridium perfringens types D and B epsilon and beta toxin genesthat was used for cloning in E.coli. The etx and cpb genes were...

background: parathyroid hormone is an 84-amino acid peptide secreted by the parathyroid glands. its physiological role is maintenance of normal serum calcium level and bone remodeling. biological activity of this hormone is related to n-terminal 1-34 amino acids. the recombinant form of hormone (1-34) has been approved for treatment of osteoporosis from 2002. in this study, a novel fusion partn...

background: sequence variations in glycoproteins of influenza virus surface impel us to design new candidate vaccines yearly. ectodomain of influenza m2 protein is a surface and highly conserved protein. m2e in influenza vaccines may eliminate the need for changing vaccine formulation every year. objectives: in this study, a recombinant baculovirus containing m2e and cholera toxin subunit b fus...

objective: chronic myeloid leukemia (cml) is a malignant clonal disorder of hematopoietic stem cells which results in increase of myeloid cells, erythroid cells and platelets in the peripheral blood and hyperplasia in bone marrow. investigations have shown different types of bcr- abl variants in these patients. in iran only 3 types of these variants have been identified because most of the clin...

Background: The Philadelphia chromosome (Ph) characterized by t (9; 22) (q34; q11.2) is a reciprocal translocation giving rise to a chimeric BCR-ABL fusion gene. Incidence of Ph chromosome is over 98% in Patients with Chronic Myeloid Leukemia (CML) and around 20% in acute lymphoblastic leukemia (ALL). The finding of this fusion gene is essential for diagnosis of CML by detection of various fusi...

introduction: reverse transcriptase-polymerase chain reaction (rt-pcr) assay is a useful tool for the detection of fusion transcript resulting from specific chromosomal translocation of the leukemia cells. a specific chromosomal abnormality, the philadelphia chromosome (ph), is present in 90% to 95% of cml patients.the aberration results from a reciprocal translocation between chromosome 9 and ...

Objective(s):To culture thein vitro mouse embryonic stem cells (mESCs) and to direct their  differentiation to germ-line cells; in present study we used a vector backbone containing the fusion construct Stra8-EGFP to select differentiated ES cells that entered meiosis.  Retinoic acid was used to differentiate embryonic stem cells to germ cells. Materials and Methods: A fragment of Stra8 gene pr...

objective: the aim of this study was to construct a pcdna3.1+ vector containing fmdv type o/irn/1/2007-vp1 gene, protein expression in bhkt7 cells and evaluation of immune response in balb/c mice. materials and methods: fmdv type o/irn/1/2007 was isolated from a cattle in ray in 2007 and serotyped. the purified vp1 gene was sub-cloned into the ptz57r/t vector and pcdna3.1+ expression vector. t...