no abstract is provided.

The rapid development of CRISPR-based gene editing has been accompanied by a polarized governance debate about the status CRISPR-edited crops as genetically modified organisms. This article argues that polarization around partly reflects failure public engagement with current state research in genomics and postgenomics. gene-editing technology become embedded narrow narrative ease precision tec...

Objective(s): Multidrug resistance (MDR) is a major obstacle in the successful chemotherapy of ovarian cancer. Inhibition of P-glycoprotein (P-gp), a member of ATP-binding cassette (ABC) transporters, is a well-known strategy to overcome MDR in cancer. The aim of this study was to investigate the efficiency and ability of CRISPR/Cas9 genome editing technology to knockdown ABCB1 gene expression ...

Gene-editing methods, particularly CRISPR, provide extraordinary opportunities for scientific insights and applications in the life sciences. However, prospects near-term to commercial forestry appear limited. Loss-of-function phenotypes that can be imparted by mutation of one or a few conserved genes offer best near term. For traits with complex inheritance, there is insufficient science guide...

conclusions our findings revealed that the zfn technology could be employed to overcome ampicillin resistance by the targeted disruption of the ampicillin resistance gene, which leads to inactivation of β-lactam synthesis. therefore, zfn technology could be engaged to decrease the antibiotic resistance issue with the construction of a zfn archive against different args. to tackle the resistance...

The traditional homologous recombination (HR) gene-editing method faces problems such as low editing efficiency and absence of marker genes. CRISPR–Cas9-editing is high has been widely used in bacteria yeast. In comparison with CRISPR–Cas9, CRISPR–Cas12a many outstanding advantages. Here, we report an Acidaminococcus sp. BV3L6 (As) Cas12a-based genome-editing for Starmerella bombicola. To demon...

Disease-causing genes have been identified for many severe muscular and neurological genetic disorders. Advances in the gene therapy field offer promising solutions drug development to treat these life-threatening conditions. Depending on how mutation affects function of product, different approaches may be beneficial. Gene replacement is appropriate diseases caused by mutations that result def...