trichophyton rubrum is an anthropophilic fungus causing up to 90% of chronic cases of dermatophytosis. several properties of this fungus have been investigated so far. however, a few studies were carried out in the field of molecular biology of this fungus. in the present study, we tried to identify the subunit g of its vacuolar atpase (v-atpase). pairs of 21 nt primers were designed from highl...

background: malassezia are dimorphic, lipid-dependent yeasts, which are responsible for causing several cutaneous and sys­temic conditions. although cyclophilins (cyps) are highly conserved cytosolic proteins that catalyze the peptidyl-prolyl cis-trans isomerazation reaction before protein folding process, it has been suggestive of an allergen in a few numbers of fungi such as aspergillus fumig...

The detection of fungal pathogens in clinical samples by PCR requires the use of extraction methods that efficiently lyse fungal cells and recover DNA suitable for amplification. We used quantitative PCR assays to measure the recovery of DNA from two important fungal pathogens subjected to six DNA extraction methods. Aspergillus fumigatus conidia or Candida albicans yeast cells were added to br...

Bacterial DNA contamination of rust fungal DNA can be a significant problem for sequencing the rust fungus. Sequence assembly is much more difficult if the sequence contigs are mixed with bacterial sequence. A quantitative real-time polymerase chain reaction (qPCR) assay was developed to quantify bacterial DNA within rust fungal DNA samples and the results were compared with those obtained from...

The reviewer appreciate the additional experiment done by the authors about DNA extraction efficiency. However, the efficiency reported in the response seems to be recovery efficiency of DNA associated purification and elution steps, and not efficiency of DNA extraction from fungal cells. In this case, the authors had to spike fungal spores, not naked fungal DNA or PCR amplicons, as an external...

Determining the etiology of invasive fungal infections (IFI) is critical for patient management as fungi vary in their susceptibility to antifungals. However, the etiology remains obscure in many cases due to negative culture results. The identification of fungal DNA by PCR in pathology blocks and sequencing it is an alternative approach to determine the cause of IFI. Previous studies identifie...

A fungus-specific PCR assay using only one primer set has been developed for detecting indoor fungi. Four fungal primer sets, NS3/NS4, NS5/NS6, FF1/FR1 and FF2/FR1, were tested with DNA from humans, rats, mice, bacteria, pollens and six commonly found fungal species (Alternaria chamydospora, Aspergillus flavus, Candida famata, Cladosporium fermentans, Penicillium chrycoIgenum and Stachybotrys c...

the aim of this study was to evaluate the effect of substitution of soybean meal with canola meal and measure its effects on rumen anaerobic fungal population in lactation iranian of holstein cows. from d 5 to 56 postpartum, cows were fed diets that were isoenergetic containing soybean meal (sbm; n = 3) or canola meal (cm; n = 3). cows were housed in tie stalls and fed tmr two times a day. comp...

There is little information on the indoor environment in hotels. Analysis of fungal DNA by quantitative PCR (qPCR) is a new method which can detect general and specific sequences. Dust was collected through swab sampling of door frames in 69 hotel rooms in 20 countries in Europe and Asia (2007-2009). Five sequences were detected by qPCR: total fungal DNA, Aspergillus and Penicillium DNA (Asp/Pe...