the reliability of gene expression profiling based technologies and methods to find transcriptional differences representative for the original samples is influenced by the quality of the extracted rna. hence, rna extraction is the first step to investigate the gene expression and its function. consequently, the quality of extracted rna is really significant. correspondingly, this research was ...

Background: Histamine is a food-borne chemical hazard which causes scombroid poisoning. The efficiency of histamine recovery from fish is greatly influenced by selection of an appropriate extraction solvent. Hence, in this study the efficiency of different extraction solvents on recovery of histamine from fresh, frozen and canned fish was evaluated. Methods: Fresh, frozen and canned rainbow tr...

background: histamine is a food-borne chemical hazard which causes scombroid poisoning. the efficiency of histamine recovery from fish is greatly influenced by selection of an appropriate extraction solvent. hence, in this study the efficiency of different extraction solvents on recovery of histamine from fresh, frozen and canned fish was evaluated. methods: fresh, frozen and canned rainbow tro...

frozen section and touch imprint cytology are important diagnostic procedures for surgeons during operation and must be accurate if the patient is to receive maximum benefit. to assess the accuracy of intraoperative pathologic consultation including frozen section and touch imprint cytology during a five year period (1995- 2000), a retrospective survey of 1000 consecutive cases of frozen sectio...

Primary tumor organoids grown in three-dimensional culture provide an excellent platform for studying tumor progression, invasion, and drug response. However, organoid generation protocols require fresh tumor tissue, which limits organoid research and clinical use. This study investigates cellular morphology, viability, and drug response of organoids derived from frozen tissues. The results dem...

Frozen section and touch imprint cytology are important diagnostic procedures for surgeons during operation and must be accurate if the patient is to receive maximum benefit. To assess the accuracy of intraoperative pathologic consultation including frozen section and touch imprint cytology during a five year period (1995- 2000), a retrospective survey of 1000 consecutive cases of frozen s...

The objective of this research is to prospectively evaluate the feasibility of vibroacoustography (VA) imaging in monitoring prostate cryotherapy in an ex vivo model. Baseline scanning of an excised human prostate is accomplished by a VA system apparatus in a tank of degassed water. Alcohol and dry ice mixture are used to freeze two prostate tissue samples. The frozen prostates are subsequently...

An experimental study of the mechanical response of frozen soft biological tissues to applied compressive stresses is presented. This study is related to the mechanical stresses that develop due to the contraction of frozen tissues in cryopreservation as well as in cryosurgical procedures. The main concept in this study is that the stresses associated with the constrained contraction of the fro...