purpose: to compare the clinical and paraclinical outcomes following deep anterior lamellar keratoplasty (dalk) using frozen versus fresh corneas in patients with keratoconus. methods: in this prospective, comparative interventional study, 16 frozen and 19 fresh corneas were used during dalk in keratoconus patients. big bubble technique was used in all patients. the two groups were compared at ...

BACKGROUND/AIMS The use of "fresh" (hypothermically stored) and frozen amniotic membrane (AM) was compared in a patient with cicatricial pemphigoid with stem cell failure. The viability of both "fresh" and frozen AM epithelial cells was assessed after storage. METHODS AM was stored at either +4 degrees C ("fresh") or at -80 degrees C (frozen). A "fresh" graft was applied to the cornea followi...

Background/aims—The use of “fresh” (hypothermically stored) and frozen amniotic membrane (AM) was compared in a patient with cicatricial pemphigoid with stem cell failure. The viability of both “fresh” and frozen AM epithelial cells was assessed after storage. Methods—AM was stored at either +4°C (“fresh”) or at −80°C (frozen). A “fresh” graft was applied to the cornea following superficial ker...

PURPOSE We investigated the expression of laminin-5 and integrins, and the ultrastructure of the interface between basal cells and the basement membrane in the keratoconus cornea. These findings were compared to those in normal central cornea and limbus. METHODS Frozen sections of the normal cornea (center and limbus) and the keratoconus cornea were immunostained with monoclonal antibodies ag...

Rabbit corneas were frozen and thawed by three methods and compared by full thickness transplantation as well as specular microscopy, histology, and transmission electron microscopy. Two of the methods used a recently described technique, in which the excised cornea was immersed in a potassium-rich buffered solution containing the cryoprotectant dimethyl sulphoxide (Me2SO, 2 mol/l). This soluti...

PURPOSE To elucidate the spatial distribution of inactivation of lactate dehydrogenase (LDH) in ultraviolet-B radiation (UVR-B)-exposed eyes. To determine the in vivo penetration depth of UVR-B in the lens. METHODS LDH activity in cornea and lens was investigated with an enzyme histochemical technique. Thirty rats were exposed in vivo to UVR-B of approximately 300 nm, and the eyes were enucle...

An improved method of banking the donor material at low temperature is described, entailing the immersion of the cornea with an attached rim of sclera in medium containing dimethyl sulphoxide. The storage temperature is that of liquid nitrogen (1960 C.) instead of that of solid carbon dioxide (79° C.), as formerly used. Though the results with deep-frozen donor material were superior to those w...

PURPOSE To study the expression of intermediate filaments in the human cornea. METHODS Light and electron microscopic and immunohistochemical studies were performed on 20 corneas from subjects of various ages. RESULTS A hitherto unrecognized epithelial cell population emerged from the immunohistochemical studies. Epithelial cells were invariably present in the superior cornea, whereas the n...