Formalin is a hazardous chemical that needs cautious handling and special storage. Owing to its disinfectant and fixative (i.e. for preserving pathologic tissue specimens in histopathology) properties, it is widely used in dentistry. Although, the terms formaldehyde and formalin are often confused as being identical, these are different as to the concentrations of the primary component i.e. for...

Recent work has cast doubt on the validity of results obtained from glutaraldehyde-fixed material in radioautography. Peters and Ashley (3) have demonstrated that glutaraldehyde binds free leucine directly onto cells of rat liver. They indicate that this bound leucine might be confused with that incorporated by protein synthesis. As an alternative fixative for radioautography, they recommend fr...

The binding of labeled free amino acids to liver and to purified protein by commonly used fixatives was investigated. Glutaraldehyde caused 25% of free leucine to be bound to serum albumin in solution, whereas formaldehyde bound only 0.5%. Liver slices were incubated for 2 min in the presence of labeled leucine and of puromycin, which permits absorption of leucine into the cell but inhibits inc...

The effects of fixation on the cell volume of marine heterotrophic nanoflagellates and planktonic ciliates were investigated. Decreases in cell volume depended on the combination of the protozoan taxa and the particular fixative. For a particular fixative and protozoan species, degree of shrinkage was independent of physiological state. The volume of fixed cells was found to be approximately 20...

This study was designed to compare tissue preservation efficiency of formaldehyde substitution (FS) on cryosections with acetone (AC) and formaldehyde (FA). Brain, kidney, heart and liver had obtained from adult Dutch rabbit and were fixed with either FA or FS and AC prior to cryosectioning. Hematoxylin and eosin staining showed that AC is a poor fixative in preserving the general tissue and ce...

Periodate-lysine-paraformaldehyde (PLP) has been proposed as a fixative for glycoprotein antigens which should stabilize periodate oxidized polysaccharide chains through lysine mediated crosslinks, either directly or by the intermediation of formaldehyde. In spite of premises and attempts reported in the literature, this fixative has never become popular for the study of membrane antigens of im...

Background: Formaldehyde is widely used in industry and in medicine as tissue fixative and disinfectant. It contains reactive molecules which have been known for its cytotoxic effects. In the present study, the probable protective properties of Rosa damacena aquatic extract, against the harmful effects of formaldehyde was evaluated by consideration of sperm count, motility, viability, morpholog...

formalin is widely used in industry and in medicine (as tissue fixative and disinfectant).it contains reactive molecules which have been known for its cytotoxic effects. to evaluate the effect of formalin exposure on the testicular tissue and sperm parameter from neonatal period through physical and sexual maturity, 28 male wister rats were assigned into two equal test and control groups. the t...