the development of culture media able to mimic the preovulatory stage of follicular environment and support nuclear and cytoplasmic maturation of oocyte is important for in-vitro fertilization (ivf) programs. it seems that the best culture media for embryonic development and cell cleavage is the natural composition which surrounds the oocyte which has been used occasionally in human ivf program...

objective: to assess follicular development and endometrial growth using transvaginal sonography in spontaneous and clomiphene citrate induced (cc) cycles. materials and methods: one hundred consecutive couples with unexplained infertility were recruited for this cross sectional study from 2003 to 2005. patients were monitored for the first cycle with no medication while the laboratory routine ...

polycystic ovarian syndrome is a hetrogenous condition with a broad clinical and pathologic spectrum that may reflect the effects of diverse etiologic factors. polycystic ovarian syndrome is an abnormality in the maturation of the follicles which can result in abnormal composition of follicular fluid. by analysis and comparison of the biochemical composition of human follicular fluid in normal ...

The aim of this study was to evaluate the effects of ovarian tissue vitrification and two-step in vitro culture on the metaphase II (MII) oocyte reactive oxygen species (ROS) level, mitochondrial transcription factor A (TFAM) expression and succinate dehydrogenase (SDH) activity. After collection of neonatal mouse ovaries, 45 ovaries were vitrified and the others (n = 45) were...

The number of ovulated oocytes is different among mammals but does not vary much within the same species. In order to sustain periodic ovulation, follicular development must be coordinated at the tissue level. Elucidating the regulatory mechanisms of follicular development is difficult because the ovary has a complicated structure and it takes a long time for primordial follicles to develop int...

Background: Administration of recombinant luteinizing hormone (rLH) in controlled ovarian hyperstimulation may benefit a subpopulation of patients. However, late follicular phase administration of high doses of rLH may also reduce the size of the follicular cohort and promote monofollicular development. Materials and Methods: To determine if rLH in late follicular development had a negative imp...

Objectives were to characterize changes in concentrations of LH, FSH, and estradiol-17beta (estradiol) in blood and populations of ovarian follicles of prepubertal cattle during waves of follicular development and to evaluate interactions between day after follicular aspiration and month prepuberty for these variables. During each month (month prepuberty), ovarian follicles of prepubertal cattl...

Ad libitum feeding causes excessive follicular development and is associated with extensive metabolic changes in broiler-breeder hens. Restricting feed intake reduces excessive follicular development, but the mechanisms mediating this response are unknown. In the present study, the effects of feeding on follicular development in immature broiler-breeder hens were examined. There was an increase...

background: fat-rich diet may alter oocyte development and maturation and embryonic development by inducing oxidative stress (os) in follicular environment. objective: to investigate the relationship between fat intake and oxidative stress with oocyte competence and embryo quality. materials and methods: in observational study follicular fluid was collected from 236 women undergoing assisted re...