a total of 781 serum and 827 red cell samples from four populations of iran (kashan, ardestan, asrara and ardabil) were sub-typed by isoelectric focusing for group-specific componet and red cell enzyme phosphoglucomutase, locus one, respectively. heterogeneity test revealed no significant differences among the populations studied regarding both pgm1 and gc systems.

A novel approach to molecular separations is investigated using a technique termed droplet-based isoelectric focusing. Drops are manipulated discretely on a superhydrophobic surface, subjected to low voltages for isoelectric focusing, and split-resulting in a preparative separation. A universal indicator dye demonstrates the generation of stable, reversible pH gradients (3-10) in ampholyte buff...

The technique previously applied to serum can, with minor modifications, be applied to cerebrospinal fluid. Good protein patterns have been obtained and similarities as well as differences have been demonstrated between cerebrospinal fluid and serum. The patterns obtained should prove useful in neurological diagnosis.

In order to examine the diversity of antibody light chains, we have developed an analytic isoelectric focusing procedure which permits the routine analysis of L chains from antibodies raised in individual mice. We have used this technique to demonstrate that the light chains of IgM and IgG anti-group A streptococcal antibodies raised in SWR mice are probably shared. Interestingly, numerous ligh...

Native Isoelectric Focusing Free-flow Electrophoresis (IEF-FFE) Fractionation of Crude Protein Mixtures Peter J.A. Weber, Gerhard Weber, and Christoph Eckerskorn This protocol was adapted from "Native IEF-FFE Fractionation of Crude Protein Mixtures," contributed by Peter J.A. Weber, Gerhard Weber, and Christoph Eckerskorn, Chapter 19, in Purifying Proteins for Proteomics (ed. Simpson). Cold Spr...

Denaturing Isoelectric Focusing Free-Flow Electrophoresis (IEF-FFE) Fractionation of Crude Protein Mixtures Peter J.A. Weber, Gerhard Weber, and Christoph Eckerskorn This protocol was adapted from "Denaturing IEF-FFE Fractionation of Crude Protein Mixtures," contributed by Peter J.A. Weber, Gerhard Weber, and Christoph Eckerskorn, Chapter 19, in Purifying Proteins for Proteomics (ed. Simpson). ...