Naphthalenic compounds are a rich resource for designers of fluorescent sensing/switching/logic systems. The degree of internal charge transfer (ICT) character in the fluorophore excited states can vary from negligible to substantial. Naphthalene-1,8;4,5-diimides (11–13), 1,8-naphthalimides (16) and 4-chloro-1,8-naphthalimides (15) are of the former type. The latter type is represented by the 4...

Terpyridine/benzofurazan conjugation results in a new hybrid fluorophore of the colorimetric sensing ability for Fe(2+) and fluorimetric sensing ability for XII group cations. The improved emission properties and cell imaging ability imply it is a suitable platform to construct a fluorescent sensor for metal imaging in biological systems.

Fluorescence diffuse imaging (FDI) suffers from limited spatial resolution. In this Letter, we report a scanning imaging approach to increase the resolution of FDI using nonlinear fluorophores. The resolution of a linear fluorophore was compared with nonlinear upconverting nanoparticles (NaYF(4):Yb(3+)/Tm(3+)) in a tissue phantom. A resolution improvement of a factor of 1.3 was found experiment...

Stimulated emission depletion (STED) resolves fluorescent features that are closer than the far-field optical diffraction limit by applying a spatially modulated light field keeping all but one of these features dark consecutively. For estimating the efficiency of transient fluorophore darkening, we developed analytical equations considering the spatio-temporal intensity profile of the STED bea...

mechanism of the binding of lomefloxacin (lmf) with human serum albumin has been studied at physiological ph (7.4) using fluorescence spectroscopic technique. lmf is a third-generation fluoroquinolone antibiotic that exhibits striking potency against a broad spectrum of gram-negative and gram-positive bacteria through inhibition of dna gyrase. lomefloxacin is a drug that is excreted in urine an...

Super-resolution optical fluctuation imaging (SOFI) is a super-resolution microscopy technique that overcomes the diffraction limit by analyzing intensity fluctuations of statistically independent emitters in time series images. The final images are background-free and show confocality enhanced spatial resolution (super-resolution). Fluorophore photobleaching, however, key limitation for record...

A general approach is presented for developing small molecule-based fluorogenic probes suitable for no-wash imaging of endogenous kinases in live cells. Probe 1, including a fluorophore-quencher system, was only "turned on" upon reacting with its target kinase Btk, and disclosed Btk's cellular location in live cells without any washing.