background and objectives: numerous procedures in biology and medicine require the counting of cells. direct enumer- ation of colony forming units (cfus) is time-consuming and dreary accurate cell counting on plates with high numbers of cfus is error prone. in this study we report a new indirect cell counting method that was developed based on the use of redsafe fluorometric assay. the usefulne...

We report a quick, easy and inexpensive fluorometric assay that measures the activity of replication enzymes using PicoGreen. The systems tested include replication of the natural template M13 Gori by E. coli DNA polymerase III holoenzyme and the replication of a synthetic homopolymer by human immunodeficiency virus reverse transcriptase. A direct comparison of the fluorometric assay with the ...

We report a quick, easy and inexpensive fluorometric assay that measures the activity of replication enzymes using Pico-GreenTM. The systems tested include replication of the natural template M13 Gori by E. coli DNA polymerase III holoenzyme and the replication of a synthetic homopolymer by human immunodeficiency virus reverse transcriptase. A direct comparison of the fluorometric assay with th...

To date, enzymatic diagnosis of Gaucher’s disease via a fluorometric assay procedure which utilizes 4-methylumbelliferyl-P-D-glucopgranoside as a substrate has not been possible when liver serves as the source of enzyme since currently employed fluorometric procedures cannot adequately differentiate between a broad-specificity fl-glucosidase and lysosomal glucocerebrosidase activities in crude ...

Three procedures have been compared for monitoring heparin in patients undergoing cardiopulmonary bypass: (1) activated clotting time (ACT) (2) protamine titration, and (3) fluorometric substrate assay. The ACT monitors the degree of anticoagulation. It is easy to perform and is relatively inexpensive, however, it does not correlate well with heparin levels and may not accurately predict the pr...

BACKGROUND AND OBJECTIVES Numerous procedures in biology and medicine require the counting of cells. Direct enumeration of Colony Forming Units (CFUs) is time-consuming and dreary accurate cell counting on plates with high numbers of CFUs is error prone. In this study we report a new indirect cell counting method that was developed based on the use of Redsafe fluorometric assay. The usefulness ...

A sensitive fluorometric assay was developed for alcohol sulfotransferase (AST). This was the first continuous fluorometric assay reported for AST. It used 3'-phosphoadenosine 5'-phosphosulfate regenerated from 3-phosphoadenosine 5'-phosphate by a recombinant phenol sulfotransferase (PST) using 4-methylumbelliferyl sulfate as the sulfuryl group donor. The recombinant PST did not use the alcohol...

background and objectives: the properties of neuraminidase produced by p. aeruginosa strain pao1 during growth in a defined medium (bhi) was examined and compared with some neuraminidase features of k. pneumoniae in this investigation. materials and methods: the enzyme was isolated from concentrated culture supernatants of p. aeruginosa which was used in a sensitive fluorometric assay by using ...