The biosynthesis of a yellow-green, fluorescent, water-soluble pigment by Pseudomonas jluorescens occurred only when the bacteria were iron-deficient and was not directly influenced by the nature of the organic carbon source. The pigment formed a very stable Fe3+ complex and was purified in this form. Pseudomonasfluorescens produced only one molecular species of fluorescent pigment; however, it...

A yellow autofluorescent pigment, generally thought to be indicative of membrane autoxidation, was found to accumulate in the retinal pigment epithelium (RPE) of rats maintained for 32 weeks on diets producing physiological antioxidant deficiency. The largest build-up of fluorescent pigment occurred in rats fed a diet high in polyunsaturated fatty acids (PUFAs) and deficient in alpha-tocopherol...

The phytopathogenic bacterium Pseudomonas syringae produces a fluorescent pigment when it is grown in iron-deficient media. This pigment forms a very stable Fe(III) complex that was purified in this form by using a novel procedure based on ultrafiltration and column chromatography. The Fe(III) complex has a molecular weight of 1,100 and contains 1 mol of Fe(III). The pigment is composed of an a...

Media and an analytical scheme have been developed which allow both a qualitative and quantitative estimation of the formation of pyocyanine, related phenazines, pyorubrin, and a blue and a yellow-green fluorescent pigment by Pseudomonas aeruginosa. Use of the defined pyocyanine medium of Frank and DeMoss with sulfate or various organic sulfur sources allowed formation of pyocyanine, related ph...

the aim of this study was to examine the effects of salinity and light intensity on the chlorophylls, ?-carotene and protein contents in dunaliella salina teod. the algae were grown in inorganic medium containing 0, 0.9, 1.8 (control), 2.6 and 3.5 m nacl under three illumination regimens [4500 (control), 9000 and 11000 lux]. the results showed that most electrophoresis protein bands were separa...

PURPOSE Orange pigment is an important sign of malignancy in melanocytic tumors. There is a question as to whether the pigment accumulation is inside of macrophages or retinal pigment epithelial (RPE) cells. We investigated which cells are involved with this color alteration. METHODS We examined enucleated specimens from two patients with choroidal melanoma and dense orange pigment on fundus ...