background cryptosporidium spp. is a widespread protozoan parasite involving humans and animals. objectives this study was carried out to evaluate the immunofluorescence assay (ifa) and pcr results for more accurate diagnosis of faecal specimens. patients and methods forty six faecal human specimens of cryptosporidium oocysts were examined by pcr and ifa in calgary, canada. in statistical analy...

objective: confocal laser scanning microscopy (clsm) is relatively a new light microscopical imaging technique with a wide range of applications in biological sciences. primary value of clsm for the biologist is its ability to provide optical sections from three-dimensional specimen. the present study was designed to assess the thickness content of in vivo accumulated dental plaque using clsm a...

conclusions the rabies virus np could be extracted and purified in an appropriate amount from infected cell culture. the results of sds-page analysis showed that the intact rabies virus np had been purified properly and thus could be used for further steps to produce the specific diagnostic rabies conjugate. results the volume of the lysate was 15 ml and it became 2.5 ml after purification, wit...

The direct immunofluorescence assay (DFA) was compared with culture for test-of-cure analysis for Chlamydia trachomatis in patients 7 to 10 days after antimicrobial therapy was given. DFA test-of-cure results correlated with culture results in 79.5% of 39 patients. Of DFA-negative patients, 97% had negative cultures. Six of seven patients with borderline DFA results had negative culture results.