Acute leukemia is a heterogeneous group of malignancies with varying clinical, morphologic, immonophenotypic, genetic and molecular characteristics. Despite the increasing importance of molecular and genetic features in the sub-classification of acute leukemias, morphologic and immunophenotypic analysis remains the main modality to diagnose acute leukemia for initial evaluation and providing a ...

The discovery of monoclonal antibodies made possible the immunological investigation of malignant hemopathies, facilitating the positive diagnosis. We are presenting several cases of acute leukaemia, chronic lymphoblastic leukaemia and one of multiple myelomas in which flowcytometry was useful for establishing the diagnosis. Today, flowcytometry represents an indispensable instrument for the ma...

Methods DCs from 21 therapy naïve (mean CD4: 256 cells/mm), 21 patients on anti-retroviral therapy (mean CD4: 342 cells/mm) and 14 healthy controls were immunophenotyped for maturation markers at baseline and after 5 hour ex vivo stimulation with TLR-4 ligand, LPS, by flowcytometry. Subsequently, the expression of SOCS-1 gene and the cytokine levels were assessed in monocytederived DCs (Mo-DC) ...

Methods Mice were sensitized (i.n.) with OVA and then received IVIg or sialic acid enriched IVIg (SA-IVIg) fragments (i.p.), and then underwent challenge (i.n.). The induction of CD4CD25Foxp3Treg was determined by flowcytometry. AHR was measured, using a flexiVent small animal ventilator. Phenotypic properties of dendritic cells (DC) from various experimental groups were assessed by flow-cytome...

Acute leukaemia of ambiguous lineage (ALAL) is a rare form of leukaemia in which morphologic, cytochemical and immuno-phenotypic features of the proliferating blasts lack sufficient evidence to classify them as myeloid or lymphoid in origin or have characteristics of both myeloid and lymphoid cells. We report a 22-year-old man presenting with clinical features of an acute lymphoblastic leukaemi...

All-trans retinoic acid (ATRA), as an active metabolite of vitamin A, has been shown to affect immune cells. This study was performed to evaluate the effect of ATRA on viability, proliferation, activation and lineage-specific transcription factors of CD4+ T cells. CD4+ T cells were separated from heparinized blood of healthy donors and were cultured in conditions, some with, some without ATRA. ...

background: ovarian cancer is the fifth leading cause of death from malignancy in women. cd4 +cd25+foxp3+ regulatory t (treg) cells are a subset of t lymphocytes with great inhibitory impact on immune response. objectives: to investigate the percentage of cd4 +cd25+foxp3+ regulatory t cells in the peripheral blood of the iranian patients with epithelial ovarian cancer compared to healthy women ...