نتایج جستجو برای: fixed in 10 formalin
تعداد نتایج: 17129008 فیلتر نتایج به سال:
in this thesis we will present three topics. we define approximate fixed points in fuzzy normed spaces. also we obtain some necessary and sufficient conditions on the existence of? -fixed points for ? > 0. at the continue some results about approximate fixed points for a class of non-expansive maps on g-metric spaces are obtained and we define approximate fixed points in partial metric spa...
abstract this study was carried out to compare disinfecting effects of nanosilver with other feed disinfectants on layer hen performance, immune system, gastrointestinal microflora and intestinal morphology. total of 90 layers (lsl) were divided into five groups consisting: 1-control, 2- formalin (200 ppm), 3- formaycin (200 ppm), 4- nanosilver in drinking water (7.5 ppm) and 5-nanosilver in f...
The use of ribonucleic acid (RNA) extracted from Hepes glutamic acid buffer-mediated organic solvent protection effect (HOPE)-fixed tissues in quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) is fairly novel. We compared qRT-PCR analysis of formalin- and HOPE-fixed, paraffin-embedded lymph node tissues from Mycobacterium bovis-infected cattle by extracting total RNA using ...
BACKGROUND The isolation of good quality DNA from routinely fixed and processed biopsy samples is crucial for the success of subsequent molecular analysis. AIMS To compare the amount of beta actin DNA extracted from upper gastrointestinal tract biopsies fixed in buffered and unbuffered formalin. METHODS Amounts of beta actin DNA extracted from forceps biopsies of the upper gastrointestinal ...
Background and Aim: Direct immunofluorescence (DIF) is represented as a gold standard method in diagnosis of autoimmune blistering dermatoses. Normal saline, liquid nitrogen and michel’ solution are a widely accepted media for preserving biopsy samples of skin or mucosa before DIF examination. Occasionally clinicians put the biopsy specimen taken for DIF in formalin 10%, occasionally clinicians...
Emphysema is characterized by enlargement of the alveoli, which is the most important parameter to assess the presence and severity of this disease. Alveolar enlargement is primarily defined on morphological criteria; therefore, characterization of this disease with morphological parameters is a prerequisite to study the pathogenesis. For this purpose, different methods of lung fixation were ev...
چکیده : آرای صادره از دادگاه ها علاوه بر شیوه های عادی از طریق فوق العاده نیز قابل اعتراض و رسیدگی مجدد هستند. در مواقعی که رای دادگاه مخل به حقوق ثالث باشد وی می تواند به ان اعتراض کند. قلمرو شمول اعتراض ثالث به تصمیمات قضایی که از آن به رای تعبیر شده محدود می گردد. ولی در مورد قرارها که به اعدادی و نهایی تفکیک می شوند، چون هیچ رویه ثابت و مشخصی در این زمینه در دادگاهها وجود نداشته و همچنین ا...
In vitro mutation artifacts after formalin fixation and error prone translesion synthesis during PCR
BACKGROUND: Clinical specimens are routinely fixed in 10% buffered formalin and paraffin embedded. Although DNA is commonly extracted from fixed tissues and amplified by PCR, the effects of formalin fixation are relatively unknown. Formalin fixation is known to impair PCR, presumably through damage that blocks polymerase elongation, but an insidious possibility is error prone translesion synthe...
background and objective: formalin-fixed paraffin-embedded tissues are a valuable source of dna for molecular studies. we designed and optimized an efficient procedure for dna extraction from formalin-fixed paraffin embedded tissues. materials and methods: seventy three blocks of cervical paraffin-embedded tissues were investigated. dna was extracted using 45 minutes boiling in alkaline solutio...
Background: An accurate histopathological assessment and reporting of testicular biopsies require an appropriate tissue fixative. We assessed the histological, histochemical, immunohistochemical quality biopsies, comparing 10% formalin versus Bouin solution as fixatives. Methods: This experimental study utilized 20 adult male albino rats equally divided into five cages for 30 days. By end exper...
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