introduction: human hematopoietic stem cells (hhscs) have been used for transplantation in hematologic failures. because the number of hhscs per cord blood unit is limited, the expansion of these cells is important for clinical application. it has been reported that cytokines and feeder layer provide a perspective to in vitro expansion of hhscs. in this regard, cord blood cd34+ cells expanded a...

Bachgroun: Hematopoietic stem cell derived from umbilical cord blood (UCB) has been used for regenerative medicine in hematological abnormalities. MicroRNAs are important regulators of gene expression that control both physiological and pathological processes such as development of tissue and cancer. Some studies have shown that miR-33, has a critical role in control of self-renewal cells. He...

bachgroun: hematopoietic stem cell derived from umbilical cord blood (ucb) has been used for regenerative medicine in hematological abnormalities. micrornas are important regulators of gene expression that control both physiological and pathological processes such as development of tissue and cancer. some studies have shown that mir-33, has a critical role in control of self-renewal cells. here...

Introduction: Human hematopoietic stem cells (hHSCs) have been used for transplantation in hematologic failures. Because the number of hHSCs per cord blood unit is limited, the expansion of these cells is important for clinical application. It has been reported that cytokines and feeder layer provide a perspective to in vitro expansion of hHSCs. In this regard, cord blood CD34+ cells ex...

Umbilical cord blood (UCB) has been used for transplantation in the treatment of hematologic disorders as a source of hematopoietic stem cells (HSCs). Because of insufficient number of cord blood CD34+ cells, the expansion of these cells seems to be important for clinical application. Mesenchymal stromal cells (MSCs), playing an important role in HSCs maintenance, were used as feeder layer. Apo...

umbilical cord blood (ucb) has been used for transplantation in the treatment of hematologic disorders as a source of hematopoietic stem cells (hscs). because of insufficient number of cord blood cd34+ cells, the expansion of these cells seems to be important for clinical application. mesenchymal stromal cells (mscs), playing an important role in hscs maintenance, were used as feeder layer. apo...

Human diploid fibroblast cells produce a spectrum of necessary growth factors and extracellular matrix (ECM) components essential for growth and proliferation of a variety of other cell types. In this study, the effect of five human embryonic fibroblast cell lines, isolated from liver, lung, skin and foreskin tissues, was investigated. A coculture system analyse was employed to cloning efficien...

The inconsistency of efficiency in murine embryonic stem cell (ESC) production might be associated with the differences in preparation and cryopreservation of the feeder cell layer. As the cryopreservation of mouse embryonic fibroblast (MEF) declined the quality of MEF as feeder cell layer, an effective protocol should be determined to produce murine ESC on frozen-thawed feeder cell layer as ef...

Background Umbilical cord blood has been used for transplantation in regenerative medicine of hematological disorders. MicroRNAs are important regulators of gene expression that control both physiological and pathological processes such as development and cancer. Some studies have shown that miR-33, p53 and c-myc have critical roles in control of self-renewal cells. Objective To understand th...

human diploid fibroblast cells produce a spectrum of necessary growth factors and extracellular matrix (ecm) components essential for growth and proliferation of a variety of other cell types. in this study, the effect of five human embryonic fibroblast cell lines, isolated from liver, lung, skin and foreskin tissues, was investigated. a coculture system analyse was employed to cloning efficien...