Effects of different concentrations of IAA (0, 0.3, 0.6 mg/l) and 2ip (0, 0.3, 0.6, 0.9, 1.2 mg/l) and their combinations on callus induction and shoot regeneration of hypocotyl and its thin cell layer (TCL) explants in tomato was studied. Explants were prepared from hypocotyls of seedlings in the aseptic condition. Hypocotyl segments were more efficient than TCL explants for callus induction a...

explants of leaves, petals, anthers, styles and ovaries of c. speciosus, c. cancellatus, c. gilanicus and c. almehensis were cultured on ls media with three different combinations of naa and bap in the dark. callus and neoformant organs (leaf and corm-like structure and ordinary and contractileroots) were fomd on some of these explants. corm explants from these species were also cultured on ms ...

in the present study, 2, 4-dichlorophenoxyacetic acid (2, 4-d) was assessed individually for its effectiveness to induce somatic embryogenesis in tea (camellia sinenesis l.). embryonic axes and cotyledons explants were dissected from the seeds. explants were cultured on murashige and skoog (ms) medium containing 0, 1 and 5 mm 2, 4-d alone for embryonic axes and 0, 1 and 5 mm 2, 4-d along with 0...

Intestinal explants are a useful model for toxicological and pathological studies, but the maintenance of tissue viability for longer periods of incubation remains challenging. The aim of this study was to evaluate the effect of age on the viability of intestinal explants. Jejunal explants were obtained from 24and 42-day-old piglets. The explants were incubated for two or four hours and were th...

An efficient protocol for callus induction and somatic embryogenesis of Cyclamen premium Mill. Was developed. In this study we investigate difference between response of two explants type leaf and petiole and two medium salt concentrations MS and 1⁄2 MS on callus percentage and somatic embryo number. Media was non-significant on callus production but leaf explants was better than petiole and in...

We investigated the effects of various concentrations of diethyl aminoethyl hexanoate (DA-6) on the regeneration and growth of adventitious buds in in vitro purple coneflower cultures. Among the 3 types of explants tested, leaf explants required higher concentrations of DA-6 than petiole and root explants in order to obtain high regeneration rates, while root explants required the lowest concen...

Explants of tobacco pith taken at various distances from the apex of a mature stem show a sharp gradient in growth potential in vitro; growth is highest in the extreme apical and basal explants, and is minimal in explants removed ca. 75 cm from the apex. Calluses produced by the vigorously growing basal explants are harder and more compact than those produced from more apical explants. The grad...

the present work developed an efficient plant regeneration and propagation system via direct organogenesis for morinda officinalis how., a valuable endangered medicinal plant. the effects of explant types on shoot induction, plant growth regulators on shoot proliferationand elongation and the subsequent rooting ability of shoots were examined. among the various types of explant, direct shoot pr...

Two explants of shell were removed from each of several fertile eggs of domestic fowl at different times during incubation. The chorioallantoic membrane (CAM) was removed from one of the explants (SHELL ONLY) and was left in situ on the other (SHELL+CAM). Explants were cultured for 24, 48 or 96 h at 37 degrees C and 5% CO2 in air in individual Petri dishes containing Dulbecco's modified Eagle's...