Introduction: A reliable source of cultured keratinocytes is essential as a component of oral mucosa substitutes to treat burns and wounds of the oral and maxillofacial region. Primary monolayer cell cultures have been also extremely helpful in the study of the basic biology and responses to stimuli, of both oral and skin keratinocytes, and many studies have used them. There are two techniques ...

objective(s):p in vitro chemosensitivity and resistance assays (csras) are a promising tool for personalized treatment of glioblastoma multiform (gbm). these assays require a minimum of 1 to 2 g of tumor specimen for testing, but this amount is not always accessible. we aimed to assess the feasibility and validity of utilizing stereotactic biopsies of gbm in csras. materials and methods: single...

Background: Staphylococci are recognized worldwide as one of the most important etiological agents of bovine mastitis due to their virulence factors such as their ability to penetrate inside mammary epithelial cells and their ability to form biofilm. Aims: The objectives of this study were to establish a model of primary mammary epithelial cells origin...

OBJECTIVE The aim of this study is the histological and immunohistochemical evaluation of ex vivo produced oral mucosal equivalents using keratinocytes cultured by direct explant technique. MATERIAL AND METHODS Oral mucosa tissue samples were obtained from the keratinized gingival tissues of 14 healthy human subjects. Human oral mucosa keratinocytes from an oral mucosa biopsy specimen were di...

Pieces of tissue were dissected from early chick and quail embryos (Stages XIII and XIV of Eyal-Giladi & Kochav, 1976; and stages 3-5 of Hamburger & Hamilton, 1951). These tissues were taken from three different regions of the early embryos, and from eight different regions of the older ones, and were derived mainly from the lower layer. Epiblast tissues were also used. The experiments were des...

PURPOSE A previous report has described an ocular surface reconstruction method involving the use of cultivated corneal epithelium derived from limbal explants. In the current study, a new culture system was developed involving the in vitro propagation on amniotic membrane (AM) of epithelial cells from enzymatically dissociated limbal epithelium. The purpose of this new method is to produce a c...

Cultured embryonic and adult skeletal muscle cells have a number of different uses. The micro-dissected explants technique described in this chapter is a robust and reliable method for isolating relatively large numbers of proliferative skeletal muscle cells from juvenile, adult or embryonic muscles as a source of skeletal muscle stem cells. The authors have used micro-dissected explant culture...

Stevia rebaudiana is a herbaceous perennial plant with great global demand due to its beneficial steviol glycosides(SGs) content. Current conventional breeding technique unable cater the need for more S. planting materials. Therefore, an improved in vitro shoot regeneration protocol was developed by using chitosan. The highest fresh weight of (0.586 ± 0.176 g/explant), dry (0.056 0.02 g/explant...