second order advantage of excitation-emission fluorescence matrix was applied for the simultaneous determination of ibuprofen and caffeine. the proposed method is based on the measurement of the native fluorescence and recording emission spectra of ibuprofen and caffeine in different excitation wavelengths. the mixture of these compounds was resolved by multivariate curve resolution coupled wit...

Second order advantage of excitation-emission fluorescence matrix was applied for the simultaneous determination of ibuprofen and caffeine. The proposed method is based on the measurement of the native fluorescence and recording emission spectra of ibuprofen and caffeine in different excitation wavelengths. The mixture of these compounds was resolved by multivariate curve resolution coupled wit...

green tea is considered as a dietary source of antioxidant nutrients, which acts upon human health. green tea leaves contain three main components in the form of simple hydroxy benzoic acids such as gallic acid, propyl gallate and xanthic bases (caffeine), have been reported to prevent or delay a number of degenerative diseases and act mainly upon the central nervous system and stimulating wake...

prediction using pure standards is expected to be biased whenever the slope of the calibration is affected by the presence of sample matrix. moreover, in the presence of unknown spectral interferents, first-order algorithms like partial least squares cannot be used. in this study, a method for determination of carvedilol (car) in tablet and urine samples is proposed by excitation-emission fluor...

in this research, gold nanoparticles were synthesized and functionalized by the antibody of aflatoxins. the quenching of the fluorescence of excitation emission matrices (eem) of two type of aflatoxins (b1, g1), provoked by the gold nanoparticles, was studied by principal component analysis (pca) and multivariate curve resolution with alternating least squares (mcr-als). these aflatoxins show q...

In This research, gold nanoparticles were synthesized and functionalized by the antibody of aflatoxins. The quenching of the fluorescence of excitation emission matrices (EEM) of two type of aflatoxins (B1, G1), provoked by the gold nanoparticles, was studied by principal component analysis (PCA) and multivariate curve resolution with alternating least squares (MCR-ALS). These aflatoxins show q...

In This research, gold nanoparticles were synthesized and functionalized by the antibody of aflatoxins. The quenching of the fluorescence of excitation emission matrices (EEM) of two type of aflatoxins (B1, G1), provoked by the gold nanoparticles, was studied by principal component analysis (PCA) and multivariate curve resolution with alternating least squares (MCR-ALS). These aflatoxins show q...

Summary Two-photon (TP) excitation (820-1150 nm) and emission (280-700 nm) spectra for the fluorescent proteins (FPs) ECFP3, EGFP3 and EYFP3 produced in human tumour cells were recorded. TP excitation spectra of pure and highly enriched samples were found to be more differentiated in comparison with their one-photon (OP) spectra. They exhibited more pronounced main and local maxima, which coinc...

This paper presents the automatic discrimination of geographical origins of milks from Western Yunnan Plateau areas and eastern China by excitation-emission fluorescence spectrometry and chemometrics. Genuine plateau milks (n = 60) and milks from eastern China (n = 89) are scanned in the regions of 180-300 nm for excitation and 200-800 nm for emission. Different options of data analysis are inv...