plant tissue culture refers to growing and multiplication of cells, tissues and organs of plants on defined solid or liquid media under aseptic and controlled environment. micropropagation allows rapid production of high quality, disease-free and uniform planting material. the micropropagation of high quality planting materials of ornamentals, and forest and fruit trees has created new opportun...

In vitro-cultured plants typically show a low photosynthetic activity, which is considered detrimental to subsequent ex vitro acclimatization. Studies conducted so far have approached this problem by analysing the biochemical and photochemical aspects of photosynthesis, while very little attention has been paid to the role of leaf conductance to CO(2) diffusion, which often represents an import...

A substantial number of micropropagated plants do not survive transfer from in vitro conditions to greenhouse or field environment. The greenhouse and field have substantially lower relative humidity, higher light level and septic environment that are stressful to micropropagated plants compared to in vitro conditions. The benefit of any micropropagation system can, however, only be fully reali...

Date palm tissue culture technique aimed to production of huge numbers of plants to farmers in order to increasing dates which have high nutritional value. Date palm plantlets from pre-acclimatization stage in vitro were transferred to acclimatize in the greenhouse cultured in peat moss + perlite 2:1 and kept in the plastic covered inside 100% humidity and 28 C0 till new leave or root was grown...

abstract large-scale mortality of in vitro raisedplantlets occurred during acclimatization, i.e. glasshouse hardening, and later at field transfer still has remained as a significant bottleneck in micropropagation. the usefulness potential of some arbuscular mycorrhizal fungi (amf) to minimize mortality rate of three commercial grape rootstock genotypes during glasshouse acclimatization was stu...