background and objectives: the selection of alternative treatment options with antibiotic combinations may be used for successful managing of multidrug-resistant acinetobacter baumannii . the aim of this study was to determine the synergistic effects of ampicillin-sulbactam combined with either levofloxacin or tigecycline against mdr a. baumannii. materials and methods: a total 124 of a.baumann...

A comparison of the Etest and the reference broth macrodilution susceptibility test for fluconazole, ketoconazole, itraconazole and amphotericin B was performed with 59 of Candida species isolated from the oral cavities of AIDS patients. The Etest method was performed according to the manufacturer's instructions, and the reference method was performed according to National Committee for Clinica...

This study correlated the daptomycin MIC results obtained by Microscan and by Etest in Staphylococcus aureus and enterococci and found that the Microscan panel GP 29 had a high rate of false nonsusceptible results. Of the isolates interpreted as nonsusceptible by Microscan, 87% of Staphylococcus aureus, 90% of Enterococcus faecalis, and 88% of Enterococcus faecium isolates were interpreted as s...

Tigecycline is often one of the few therapeutic options for infections by multidrug-resistant Gram-negative bacteria. Therefore, accurate MIC testing is critical. Since in many laboratories tigecycline is not included in the routine panel of antibiotics for susceptibility testing, tigecycline MICs are frequently determined by Etest. However, previous studies have shown that tigecycline MICs det...

BACKGROUND The aim of this study was to determine the distribution of vancomycin and daptomycin MICs among methicillin-resistant Staphylococcus aureus (MRSA) blood isolates, the prevalence of heterogeneous vancomycin-intermediate S. aureus (hVISA) and the relationship between hVISA and vancomycin MIC values. METHODS A total of 175 MRSA blood isolates were collected from seven university hospi...

The main objective of this investigation is to find out the minimum inhibitory concentration (MIC) of Staphylococcus aureus with reduced inclination to vancomycin among 30 clinical isolates of methicillin-resistance Staphylococcus aureus (MRSA) and was screened by Etest and broth dilution methods. The comparative analysis of MIC for vancomycin by broth dilution and Etest methods discloses that ...

Vancomycin and daptomycin MICs from 161 isolates of methicillin-resistant Staphylococcus aureus (MRSA) were compared using commercial and in-house broth microdilution, Etest, and common automated methods. Vancomycin Etest MICs were higher than those of other methods, whereas the MICs for daptomycin testing were comparable. Vancomycin MICs vary depending on the testing methodology.

This study examined the in vitro effects of polymyxin B, tigecycline, and rifampin combinations on 16 isolates of extensively drug-resistant Acinetobacter baumannii, including four polymyxin-resistant strains. In vitro synergy was demonstrated in 19 (40%) of a possible 48 isolate-antibiotic combinations by time-kill methods, 8 (17%) by checkerboard methods, and only 1 (2%) by Etest methods. The...

The performance of the Etest and agar dilution for in vitro antimicrobial susceptibility of Campylobacter spp. was evaluated using a quality control strain Campylobactor jejuni ATCC 33560, and 81 C. jejuni and 54 Campylobacter coli isolates recovered from retail raw meats. Seven antimicrobial agents: chloramphenicol, ciprofloxacin, doxycycline, erythromycin, gentamicin, nalidixic acid and tetra...

We studied whether the Etest can be used as an alternative to agar dilution to determine antimicrobial susceptibilities of ceftriaxone, cefixime, and cefpodoxime in Neisseria gonorrhoeae surveillance. One hundred fifteen clinical and laboratory isolates of N. gonorrhoeae were tested following the Clinical Laboratory Improvement Amendments (CLIA)-approved CLSI standard agar dilution method and, ...