نتایج جستجو برای: esat 6 protein

تعداد نتایج: 2065964  

Journal: :iranian journal of immunology 0
azam torabi department of animal science, faculty of agriculture, ferdowsi university of mashhad mojtaba tahmoorespur department of animal science, faculty of agriculture, ferdowsi university of mashhad fatemeh vahedi razi vaccine and serum research institute, mashhad nader mosavari department of ppd and tuberculin production, razi vaccine and serum research institute, karaj, iran mohammadreza nassiri department of animal science, faculty of agriculture, ferdowsi university of mashhad

background: tuberculosis is a disease with high morbidity, caused mainly by mycobaterium tuberculosis (m.tb.). dna vaccines show a promising future due to their unique advantages over conventional methods. the early-secreted antigen target (esat)-6 and culture filtrate protein (cfp)-10 of m.tb. antigens have been identified as vaccine candidates against mycobacteria and used as subunit vaccines...

Azam Torabi Fatemeh Vahedi, Mohammadreza Nassiri Mojtaba Tahmoorespur Nader Mosavari

Background: Tuberculosis is a disease with high morbidity, caused mainly by Mycobaterium tuberculosis (M.tb.). DNA vaccines show a promising future due to their unique advantages over conventional methods. The early-secreted antigen target (ESAT)-6 and culture filtrate protein (CFP)-10 of M.tb. antigens have been identified as vaccine candidates against Mycobacteria and used as subunit vaccines...

A. Rezaei Mokaram A. Zavaran Hoseini E. Asli M. Taghizadeh M. Tebianian S.M. Ebrahimi

  The identification of a large number of antigens with potential for development of new tuberculosis vaccine has been accomplished in recent years. This study was designed for cloning and expression of ESAT-6 as a potent antigen of Mycobacterium tuberculosis. Selected gene (Rv3875) was amplified by PCR and product was ligated into expressing plasmid vector pQE30 and recombinant pQE30-ES plasmi...

Journal: :iranian journal of medical sciences 0
atefeh seghatoleslam recombinant protein laboratory, biochemistry department, medical school, shiraz university of medical sciences, shiraz, iran; and histomorphometry and stereology research center, medical school, shiraz university of medical sciences, shiraz, iran mina hemmati recombinant protein laboratory, biochemistry department, medical school, shiraz university of medical sciences, shiraz, iran; and department of biochemistry, faculty of medicine, birjand university of medical sciences, birjand, iran saeedeh ebadat recombinant protein laboratory, biochemistry department, medical school, shiraz university of medical sciences, shiraz, iran; bahram movahedi recombinant protein laboratory, biochemistry department, medical school, shiraz university of medical sciences, shiraz, iran; zohreh mostafavi-pour recombinant protein laboratory, biochemistry department, medical school, shiraz university of medical sciences, shiraz, iran; and maternal-fetal medicine research center, shiraz university of medical sciences, shiraz, iran

background: macrophage immune responses are affected by the secretory proteins of mycobacterium tuberculosis (mtb). this study aimed to examine the immune responses of macrophages to mtb secretory antigens, namely esat-6, cfp-10, and esat-6/cfp-10. methods: thp-1 cells (a human monocytic cell line) were cultured and differentiated to macrophages by phorbol 12-myristate 13-acetate. the cytotoxic...

Objective(s): Tuberculosis (TB) is still one of the problematic infectious diseases in developing countries, especially in Iran. In the present study, we applied ribosome display technique to select single chain variable fragments (scFvs) specific for the 6-kDa early secretory antigenic target (ESAT-6) antigen of Mycobacterium tuberculosis from a mouse scFv library. Materials and Methods: The g...

ابراهیمی, سید محمود, امینی, یوسف, تبیانیان, مجید, دباغیان, مهران, فصیحی رامندی, مهدی, مصوری, نادر, نجمی نژاد, حمید , یعقوبی, سجاد ,

Introduction: Among several tuberculosis vaccine candidates for replacement of BCG, ESAT-6 protein has a special role. Since mycobacterium tuberculosis infection most often attacks the lungs, intranasal rout can be regarded as appropriate methods for tuberculosis vaccines and drug delivery. One of the appropriate systems for intranasal vaccine delivery is using biodegradable nanoparticles. Amon...

Journal: :archives of razi institute 2016
e. asli m. taghizadeh a. rezaei mokaram s.m. ebrahimi a. zavaran hoseini

the identification of a large number of antigens with potential for development of new tuberculosis vaccine has been accomplished in recent years. this study was designed for cloning and expression of esat-6 as a potent antigen of mycobacterium tuberculosis. selected gene (rv3875) was amplified by pcr and product was ligated into expressing plasmid vector pqe30 and recombinant pqe30-es plasmid ...

Journal: :Molecular medicine 2003
Donatella Vincenti Stefania Carrara Patrizia De Mori Leopoldo P Pucillo Nicola Petrosillo Fabrizio Palmieri Orlando Armignacco Giuseppe Ippolito Enrico Girardi Massimo Amicosante Delia Goletti

The early secretory antigenic target (ESAT)-6 purified protein and peptides from Mycobacterium tuberculosis were evaluated as antigens for the immunodiagnosis of tuberculosis (TB). Because the control of TB requires improved diagnostic procedures, efforts have increased to identify Mycobacterium tuberculosis-specific epitopes for the immunodiagnosis of active TB and to discriminate between acti...

Journal: :Journal of bacteriology 2004
Limei Meng Okkels Peter Andersen

In the present study, we demonstrate that, in analogy with the genes encoding ESAT-6 and CFP-10, the genes rv0287 and rv0288 from the ESAT-6 gene family are cotranscribed. Using Western-Western blotting and protein-print overlay methodologies, we demonstrate that ESAT-6 and CFP-10, as well as the protein pair Rv0288/Rv0287, interact pairwise in a highly specific way. Most notably, the ESAT-6 pr...

2017
Bock-Gie Jung Xisheng Wang Na Yi Justin Ma Joanne Turner Buka Samten

As early secreted antigenic target of 6 kDa (ESAT-6) of Mycobacterium tuberculosis (Mtb) is an essential virulence factor and macrophages are critical for tuberculosis infection and immunity, we studied ESAT-6 stimulated IL-6 production by macrophages. ESAT-6 stimulated significantly higher IL-6 secretion by murine bone marrow derived macrophages (BMDM) compared to culture filtrate protein 10 k...

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