fascioliasis is a worldwide parasitic disease in human and domestic animals. the causative agents of fascioliasis are fasciola hepatica and fasciola gigantica. in the recent years, fasciola resistance to drugs has been reported in the many of publications. fasciola spp has detoxification system including gst enzyme which may be responsible for its resistance. therefore , the aim of the study wa...

This study analyzed the inhibition activity of Swietenia mahagoni seeds extract on α-glucosidase and α-amylase enzymes inhibition assays.  Swietenia mahagoni seeds were extracted by using Supercritical Carbon Dioxide (SC-CO2) extraction at pressures of 20- 30 MPa and temperatures of 40- 60°C.  The oil yields obtained were analyzed with α- glucosidase and α- amylase enzymes inhibition assays.  A...

Peptide-based passivation strategies were developed to prevent the unintended conjugation of his-tagged enzymes quantum dot (QD) biosensors, enabling application QD biosensors in complex assays such as CRISPR-based diagnostics.

and spleen systems cannot be attributed to lack of precision of the assays (p = 0.01). We analyzedpurifiedliverferritin at several concentrations by immunoradiometric assay, using the spleen system, and similarly analyzed purified spleen fernitin, using the liver system. When theresultsforthepurifiedferntins were plotted along with the standards used in the analyses, the two fernitins appeared ...

Laccases are useful biocatalysts for many diverse biotechnological applications. In this study we have established efficient and reliable expression systems and high-throughput screenings for the recombinant CotA-laccase from Bacillus subtilis. The expression levels of cotA-laccase were compared in five different Escherichia coli host strains growing in 96-well microtiter plates under different...

Biological assays at the single molecule level are crucial to fundamental studies of DNA-protein mechanisms. In order to cater for high throughput applications, one area of immense research potential is single-molecule bioassays where miniaturized devices are developed to perform rapid and effective biological reactions and analyses. With the success of various emerging technologies for enginee...

This study aimed to evaluate the antioxidant and hepatoprotective effects of aqueous 60% ethanol extracts Allium ochotense Prokh. against alcohol-induced cytotoxicity as well on activities alcohol-metabolic enzymes. Antioxidant were analyzed using 3-ethylbenzothiazoline-6-sulfonic acid, 1,1-diphenyl-2-picrylhydrazl, ferric reducing power, malondialdehyde assays, found that both exhibited consid...