in this article, the immobilization of microsomal membranes on fractosil and hexadecylfractosil by hydrophobic adsorption is reported. microsomes were prepared from rat brain and thecatalytic activity of antimycin a insensitive nadh-cytochrome c reductase (nccr), one of themembrane bound enzymes in the microsomal electron transport chain, was chosen as a representative ofthe microsomal membrane...

probable use of chemical and biochemical detergents to wash polysulphone membranes using in milk ultrafiltration needs a multidisciplinary investigation. by increasing use of these membranes in food processing, washing and recovery of initial permeate flux, in order to use for a longer time from this technology, has much importance. in this research, influence of chemical detergents including e...

The glucose oxidase (GOx) enzyme was immobilized on chitosan-based porous composite membranes using a covalent bond between GOx and the chitosan membrane. The chitosan-based porous membranes were prepared by the combination of the evaporation- and non-solvent-induced phase separation methods. To increase the membrane conductivity, carbon nanotubes (CNTs) were added to the chitosan solution. The...

The interaction of soluble tyrosine hydroxylase with isolated chromaffin granule membranes was studied. The incubation of the granule membranes with the soluble fraction in the mixture that may approximate the intracellular environment of the resting cell resulted in a marked increase in the enzyme activity recovered in the membrane fraction, and enzyme activity precipitated with the granule me...

We previously purified a cytosolic phospholipase A1 that could catalyze the preferential hydrolysis of phosphatidic acid in mixed-micelle assays. Here we studied the enzyme's interactions with unilamellar lipid membranes and examined effects of the lipids on enzyme binding, stability, and catalysis. A major finding was that membrane lipids could influence the stability, activity, and specificit...