despite the availability of various alkaline phosphatase (alp) isoenzymes, the calf enzyme is being used in current enzyme assays as the detector enzyme. the glycosylation pattern of this enzyme makes it a suitable ligand for binding to wheat germ agglutinin lectin (wga). as a result of this property, the enzyme can not be used as a conjugate with this lectin, and the calf enzyme conjugates can...

Abstract Sensitive DNA assays are of importance in life science and biomedical engineering, but they heavily dependent on thermal cycling programs or enzyme-assisted schemes, which require the utilization bulky devices costly reagents. To circumvent such requirements, we developed an isothermal enzyme-free sensing method with dual-stage signal amplification ability based coupling use catalytic ...

Background: Taq DNA polymerase is a very important enzyme for molecular biological studies such as DNA amplification and DNA sequencing by the PCR. It is a standard enzyme that is used in 90% of molecular biology labs today. The aim of this study was to produce Taq DNA polymerase enzyme in E. coli by a reliable, practical, simple and low cost method. Materials and Methods: In this study, the T...

introduction: different isolates of human specifies, giardia intestinalis, also known as giardia lambia, have been shown to vary in sensitivity to drug, isoenzyme pattern, virulence and infectivity, antigens and restriction enzyme pattern. material and methods: using system that distinguishes sequence differences in the metabolic enzyme tpi, dna extracted from trophozoite that culture in modifi...

Nucleic acid amplification testing (NAAT) is the gold standard for infectious disease diagnostics. Currently NAATs are mainly limited to centralized laboratories, while paper-based antigen tests used rapid home-based DNA extraction, initial sample preparation step in NAATs, remains a bottleneck that hinders its development toward kits. This requires use of compounds detrimental enzymes downstre...

e-MagnetoMethyl IP is a new method for electrochemical analysis of global DNA methylation. It avoids bisulfite treatment, PCR amplification, and enzyme-based signal generation can detect differences as low 5% in methylation levels.

Despite the availability of various alkaline phosphatase (ALP) isoenzymes, the calf enzyme is being used in current enzyme assays as the detector enzyme. The glycosylation pattern of this enzyme makes it a suitable ligand for binding to wheat germ agglutinin lectin (WGA). As a result of this property, the enzyme can not be used as a conjugate with this lectin, and the calf enzyme conjugates can...

Molecular beacons represent a new family of fluorescent probes for nucleic acids, and have found broad applications in recent years due to their unique advantages over traditional probes. Detection of nucleic acids using molecular beacons has been based on hybridization between target molecules and molecular beacons in a 1:1 stoichiometric ratio. The stoichiometric hybridization, however, puts ...

Tumor-specific miRNA imaging is achieved through the integration of enzyme-triggered catalytic hairpin assembly with lipid-nanoparticle-based delivery. The approach enables controlled activation signal amplification in cancer cells improved contrast, as reported by Lele Li, Mengyuan and co-workers their Research Article (e202214230).