Plant cell walls represent an abundant, renewable source of biofuel and other useful products. The major bottleneck for the industrial scale-up of their conversion to simple sugars (saccharification), to be subsequently converted by microorganisms into ethanol or other products, is their recalcitrance to enzymatic saccharification. We investigated whether the structure of pectin that embeds the...

During the past decades, bioethanol becomes the best alternative to fossil fuels. Ethanol production by using edible feedstocks like sugarcane and grains became a point of concern in terms of the food supply and demand. Lignocellulosic biomass comprises non-edible feedstock opened a new method for the second-generation bioethanol production. Bioethanol production from lignocellulosic biomass is...

The mixed microbial communities that occur naturally on lignocellulosic feedstocks can provide feedstock-specific enzyme mixtures to saccharify lignocelluloses. Bacterial-fungal communities were enriched from switchgrass bales to deconstruct ammonia-pretreated switchgrass (DSG). Correlation analysis was carried out to elucidate the relationship between microbial decomposition of DSG by these co...

sugar beet pulp is one of the sugar industries by-products which can be used for cellulase enzyme production, enzymatic saccharification, and alcohol production due to its high percentage of lingo-cellulosic content. trichoderma spp. is an important fungus that produces a wide range of cellulytic enzymes. in this study, cellulase enzyme was produced by placing sugar beet pulp in trichoderma fer...

Production of advanced biofuels, green chemicals, and bio-based materials from renewable lignocellulosic biomass would contribute to decreased dependence on fossil resources and to sustainable development. The overall aim of the investigations was to explore how preprocessing and pretreatment technologies affected the chemical composition of cellulosic materials and their susceptibility to enzy...

This article reviews developments in the technology for ethanol production from lignocellulosic materials by “enzymatic” processes. Several methods of pretreatment of lignocelluloses are discussed, where the crystalline structure of lignocelluloses is opened up, making them more accessible to the cellulase enzymes. The characteristics of these enzymes and important factors in enzymatic hydrolys...

UNLABELLED BACKGROUND Nonspecific (nonproductive) binding (adsorption) of cellulase by lignin has been identified as a key barrier to reduce cellulase loading for economical sugar and biofuel production from lignocellulosic biomass. Sulfite Pretreatment to Overcome Recalcitrance of Lignocelluloses (SPORL) is a relatively new process, but demonstrated robust performance for sugar and biofuel ...

Ultrafiltration reactors based on polymeric or ceramic membranes were shown to be suitable catalytic systems for fast enzymatic saccharification of cellulose, allowing the full recovery and reuse of enzymes. By pre-treating cellulose with the IL 1-butyl-3-methylimidazolium chloride, the suitability of this substrate for enzymatic saccharification in a reactor based on polymeric ultrafiltration ...

Red maple, sweet gum, trembling aspen, red alder, and Eucalyptus globulus samples were pretreated with dilute sulfuric acid and green liquor before enzymatic saccharification. Substrates showed different levels of delignification and sugar recovery, depending on the applied pretreatments and the syringaldehyde/vanillin ratio (S/V). Three major conclusions were drawn in this research. First, lig...

The optimization of biomass loading enzyme loading, surfactant concentration and incubation time, using response surface methodology (RSM) and Box Behnken design for enzymatic saccharification of sugarcane tops (SCT) for maximum recovery of fermentable sugars using crude cellulases, resulted in 90.24% saccharification efficiency. Maximum saccharification yield of 0.376 g/g glucose as substrate ...