production of lignocellulolytic enzymes by the thermotolerant fomes sp. eum1 was determined in solid cultures using corn stover (cs) as a sole substrate or supplemented with 20 % wheat bran (cs+wb). this supplementation increased (p< 0.05) enzymatic activity per gram of initial dry matter (gdm) for xylanases and cellulases: 160 iu g dm-1 and 37 iu g dm-1, respectively; while laccases reached a ...

The sole energy-yielding reaction of the autotrophic nitrifying bacterium Nitrosomonas europaea involves the conversion of ammonium to nmtrite. As early as 1926, Kluyver and Donker postulated that the six electron oxidation proceeds with the intermediary formation of hydroxylamine and hyponitrous acid. Only recently have there been any studies of the biochemical mechanism of chemoautotrophic am...

Potential antioxidative activities of enzymatic extracts from seven species of brown seaweeds were evaluated using four different reactive oxygen species (ROS) scavenging assays containing DPPH (1,1-diphenyl-2-pricrylhydrazyl) free radical, superoxide anion, hydroxyl radical and hydrogen peroxide scavenging assay. The brown seaweeds were enzymatically hydrolyzed to prepare water-soluble extract...

The objective of this study was to characterize the non-enzymatice and enzymatic antioxidants of E. littorale Blume grown under wild condition. Aerial and sub-aerial parts of the plants exposed to different heat stress was analyzed for the non enzymatic antioxidants such as Tocopherol, Phenols, DPPH, FRAP and enzymatic antioxidants such as Polyphenol oxidase, Peroxidase, Catalase, Super Oxide D...

We describe a method for determining free and total cholesterol in extracts of rat-liver lipids by use of a cholesterol esterase/cholesterol oxidase enzymatic kit (Boehringer Mannheim Corp). The lipids are solubilized (as micelles) directly into the aqueous reagent mixture. Results of the enzymatic method correlate well with those obtained by an acid/ferric chloride assay of digitonin precipita...

a vast majority of the studies addressing the free radicals including hydroxyl radical is a damage compound of biochemical molecules such as dna, proteins and lipids. when free radicals specially hydroxyl radical are not adequately removed from the body, it may damage biological macromolecules, leading to a variety of disease occurs. therefore, the body should be protected by an enzymatic or no...

Saccharomyces cerevisiae (S. cerevisiae) (commonly known as baker's yeast) is a model organism that has a similar upstream base excision repair (BER) pathway for the repair of methylated bases as that in mammalian cells, and it is very easy to maintain in the laboratory environment. Here, we described a method to prepare cell extracts from yeast to investigate their enzymatic activities. This p...