GRP94 (gp96), which performs established functions as a molecular chaperone and immune system modulator, has been reported to display a number of intrinsic enzymatic activities, including ATP hydrolysis, protein phosphorylation, and aminopeptidase. In observing that GRP94 co-purified with bacterial -galactosidase through multiple chromatographic steps, we have examined the hypothesis that the r...

N.sitophila was grown under controlled conditions of solid state and submerged fermentation on Sago hampas. The optimum conditions of protein enrichment previously established for sugar beet pulp was used for this study. Under this condition the protein content of Sago hampas under solid state increased from 1.4 to 14.45% (W/W) whereas for Sago hampas and Sago starch, the protein content under ...

in this study the bacterial growth inhibitory activity of peptide fragments produced from goat’s milk whey proteins by enzymatic hydrolysis using trypsin, ficin and a combination of both was investigated. goat’s milk whey proteins were isolated and subjected to enzymatic hydrolysis and peptides were purified by ultrafiltration followed by reverse-phase high-performance liquid chromatography (rp...

Between 1930 and 1975 biochemical and structural analysis of enzymes led to a clear set of ideas that might form a basis for detailed understanding of enzyme action. Further development required energetic and thermodynamic analysis of enzymes in an aqueous medium, beyond the computational power then available. Structural enzymology advanced in other directions, but the fundamental questions of ...