objective: the role of staphylococcal enterotoxin b (seb) in food poisoning is well known, however its role in other diseases remains to be explored. the aim of this study is the molecular screening and characterization of the seb gene in clinically isolated strains. materials and methods: in this experimentally study, 300 staphylococcus aureus (s. aureus) strains isolated from clinical samples...

Background: In this study we aimed at detecting the enterotoxin A and B gene of S. aureus in clinical samples of patients attending health centers in Zahedan using molecular methods. Materials and Methods: A cross-sectional study was carried out in which 40 samples of S. aureus were obtained from patients in a hospital in Zahedan, Iran. Following the biochemical tests, Identifications were c...

staphylococcus aureus is a hazard to human health since they can cause a wide variety of hospital-associated infections ranging from minor skin infections to post-operative wound infections and food poisoning which produces many different virulence factors, including enterotoxins (ses). although  studies  have  been  done  regarding  the difference  between  virulence  factors  of  sensitive st...

background: in this study we aimed at detecting the enterotoxin a and b gene of s. aureus in clinical samples of patients attending health centers in zahedan using molecular methods. materials and methods: a cross-sectional study was carried out in which 40 samples of s. aureus were obtained from patients in a hospital in zahedan, iran. following the biochemical tests, identifications were conf...

OBJECTIVE The role of staphylococcal enterotoxin B (SEB) in food poisoning is well known, however its role in other diseases remains to be explored. The aim of this study is the molecular screening and characterization of the SEB gene in clinically isolated strains. MATERIALS AND METHODS In this experimentally study, 300 Staphylococcus aureus (S. aureus) strains isolated from clinical samples...

OBJECTIVES To investigate the potential use of DNA techniques in epidemiological diagnosis of Bacillus cereus food poisoning. SUBJECTS Fifty six B. cereus isolates from milk were studied. DESIGN The 56 B. cereus isolates were characterised into enterotoxin positive (27 isolates) and enterotoxin negative (29 isolates) using reverse passive latex agglutination technique. SETTING Plasmid and...

Out of 321 strains of Bacillus cereus from several sources and isolated in four different countries, 239 (74%) produced cytotoxins. Only 127 (53%) of the cytotoxic strains were positive for the B-component gene of the haemolysin BL (enterotoxin) by polymerase chain reaction (PCR). Western blots using antiserum produced against enterotoxin(s) gave positive results for 199 (83%) of the cytotoxic ...

Background: Clostridium perfringens is known as the most widely distributed pathogenic microorganism in nature. It is an extremely important pathogen of human and domestic animals. In a commonly used classification scheme, C. perfringens is divided into five toxinotypes (A to E) based on the production of four major toxins (alpha, beta, epsilon, and iota). Enterotoxin is not usually used for C....

Bacteroides fragilis constitutes about 1% of the bacterial flora in intestines of normal humans. Enterotoxigenic strains of B. fragilis have been associated with diarrheal diseases in humans and animals. The enterotoxin produced by these isolates induces fluid changes in ligated intestinal loops and an in vitro cytotoxic response in HT-29 cells. We developed a nested PCR to detect the enterotox...