many materials have been introduced for apexification each having their own advantages and disadvantages. this case report aims to present a new method of apexification using a combination of deproteinized bovine bone mineral (dbbm) and enamel matrix derivative (emd). after irrigating the canal of the maxillary right canine with 2.5 % sodium hypochlorite, a mixture of bio-oss and emd was packed...

background: the aim of this clinical trial study was to clinically evaluate the use of enamel matrix protein derivative combined with the coronally positioned fl ap to treat gingival recession compared to the subepithelial connective tissue graft by a new method to obtain denuded root surface area. materials and methods: thirteen patients, each with two or more similar bilateral miller class i ...

Enamel crystallites form in a protein matrix located proximal to the ameloblast cell layer. This unique organic extracellular matrix is constructed from structural protein components biosynthesized and secreted by ameloblasts. To date, three distinct classes of enamel matrix proteins have been cloned. These are the amelogenins, tuftelin, and ameloblastin, with recent data implicating ameloblast...

The extracellular tooth enamel matrix is a unique, protein-rich environment that provides the structural basis for the growth of long and parallel oriented enamel crystals. Here we have conducted a series of in vivo and in vitro studies to characterize the changes in matrix shape and organization that take place during the transition from ameloblast intravesicular matrices to extracellular subu...

The Ras-related C3 botulinum toxin substrate 1 (Rac1) gene encodes a 21-kDa GTP-binding protein belonging to the RAS superfamily. RAS members play important roles in controlling focal adhesion complex formation and cytoskeleton contraction, activities with consequences for cell growth, adhesion, migration, and differentiation. To examine the role(s) played by RAC1 protein in cell-matrix interac...

The secreted, full-length amelogenin is the dominant protein of the forming enamel organ. As enamel mineralization progresses, amelogenin is quickly subjected to proteolytic activity, and eliminated from the enamel environment. Mature enamel contains only traces of structural proteins, including enamelin and the sheath protein ameloblastin. In addition, a proteolytic fragment of amelogenin, kno...

Amelogenesis Imperfecta (AI) can be caused by the deficiencies of enamel matrix proteins, molecules responsible for the transportation and secretion of enamel matrix components, and proteases processing enamel matrix proteins. In the present study, we discovered the double deletion of bone morphogenetic protein 2 (Bmp2) and bone morphogenetic protein 4 (Bmp4) in the dental epithelium by K14-cre...

Unlike other mineralized tissues, mature dental enamel is primarily (> 95% by weight) composed of apatitic crystals and has a unique hierarchical structure. Due to its high mineral content and organized structure, enamel has exceptional functional properties and is the hardest substance in the human body. Enamel formation (amelogenesis) is the result of highly orchestrated extracellular process...

The organic matrix of dental enamel is produced by morphologically characteristic cells of the enamel organ (ameloblasts), which are derived from the stratified epithelial cells of the primitive oral cavity (1). On the basis of its ectodermal origin, as well as its chemical composition, physical properties, and histochemical staining characteristics, the protein component of the organic matrix,...