a mathematical model of sorption of zirconium and hafnium cations mixture and elution of these ions from a cation exchange resin bed is developed. sulfuric acid is selected as the eluent for selective separation of zr and hf in a packed bed of dowex cation exchange resin. the column model is a dispersed plug flow model,and assumed to consist of randomly packed synthetic and spherical resin bead...

the aim of this study was to assess the effect of bleaching on elution of monomers from nanofilled and microhybrid composites.80 samples (5mm diameter and 3mm thickness) of each composite were prepared. after curing, half of them were randomly polished. each group was divided into 8 subgroups and immersed in water or 10%, 20% and 30% h2o2 for 3 or 8 hours. eluted bis-gma (bis-phenol a glycidyl ...

A mathematical model of sorption of Zirconium and Hafnium cations mixture and elution of these ions from a cation exchange resin bed is developed. Sulfuric acid is selected as the eluent for selective separation of Zr and Hf in a packed bed of Dowex cation exchange resin. The column model is a dispersed plug flow model,and assumed to consist of randomly packed synthetic and spherical resin ...

With so many options for method parameters to adjust during development, identifying a starting point can be intimidating. Starting with scouting gradients simplify the process, and yield rich results that inform subsequent development steps. In this installment, we discuss how design gradients, use resulting data make decisions about next steps (including whether isocratic or gradient elution)...

This study provides a detailed investigation into the performance of a stimuli responsive ELP-Z based process for monoclonal antibody (mAb) affinity precipitation. A multidimensional high-throughput screening (HTS) protocol was developed and employed to investigate the effects of a variety of operating conditions on mAb yield and aggregation during the process. Precipitation efficiency of ELP-Z...

Poliovirus-seeded tap water, conditioned with MgCl2 and passed through virus-adsorbing filters, gave better poliovirus recovery than water identically treated but conditioned with AlCl3. Elution of several filter types with beef extract yielded higher recoveries than did elution with glycine. Seeded samples filtered through various filters and stored showed considerable virus loss in 2 days whe...

In obese women (a = 16) at their usual weight, fasting adipose tissue lipoprotein lipase (LPL) activity, obtained by elution with serum and heparin at 40 and 370C, was inversely correlated to plasma estradiol levels (r = -0.724; P = 0.002) and (r = -0.641; P = 0.010), respectively. Furthermore, fasting postheparin plasma LPL activity during a heparin infusion, showed an even stronger inverse co...

DNA fragments that show a pattern of differential expression on differential display gels must be eluted from the gel matrix and reamplified to enable further analysis. Elution is usually achieved by heating excised gel slices in a small volume of either water or TE. Here we show that this elution step can adversely affect the ability of the eluted DNA to act as a template for PCR reamplificati...