introduction: iache (acetylcholinesterase) is one of the plasma’s enzymes that plays a crucial role in nervous signal transduction in synapses and is responsible for the hydrolysis of ach. material & method: we investigated three methods for the determination of ache in human blood serum, using three different assays. michel methods are based on the measurement of hydrogen ion produced in proce...

Introduction: IAChE (acetylcholinesterase) is one of the plasma’s enzymes that plays a crucial role in nervous signal transduction in synapses and is responsible for the hydrolysis of Ach. Material & Method: We investigated three methods for the determination of AChE in human blood serum, using three different assays. Michel methods are based on the measurement of hydrogen ion produced in pro...

This method is author’s innovative technique which follows number of applied surgical procedures on palate and uvula with only goal to advance functional results and avoid complications like velofaringeal insufficiency. Task of this method is to solve uvulopalato lateral obstruction and to expand oropharyngeal airway. It was introduced in the year 2000 by Dr. Vukoje. It requires strict indicati...

An electrochemical sensor is introduced as a tool applicable for diagnosis of intoxication by cholinesterase inhibitors caused by the well-known nerve agent VX. The traditional Ellman method was chosen for comparison with the sensor's analytical parameters. Both methods are based on estimation of blood cholinesterase inhibition as a marker of intoxication. While Ellman´s method provided a limit...

Organophosphorus compounds (OPs) are potent inhibitors of acetylcholinesterase (AChE). Treatment for OP poisoning is by administration of atropine sulfate, an oxime, and diazepam. Oximes such as 2-PAM are used to reactivate OP-inhibited AChE so as to restore normal enzymatic function and serve as a true antidote. There are reports of non-enzymatic hydrolysis by oximes of acetylthiocholine in in...

OBJECTIVE To compare the efficacy and safety of four radiofrequency generators (Ellman, Select Sutter, Coblator, Somnus) for the treatment of simple snoring. MATERIALS AND METHODS Multicenter, randomized, prospective single-blind study on 120 selected patients with simple snoring (apnea/hypopnea index <10/h of sleep). Snoring sound intensity was measured on a visual analog scale and the partn...

We describe here a field method we developed for colorimetry of erythrocytic acetylcholinesterase (EC 3.1.1.7) in capillary blood samples. Three stable, premixed assay reagents and de-ionized water (but no centrifuge or balance) are required. This method, adapted for a microplate format, is essentially that of Ellman et al. (Biochem Pharmacol 1961;7:88-95) as modified by George and Abernethy (C...

This paper presents a protocol for routine assays of human blood cholinesterase activities which separates erythrocytes from plasma by centrifugation and measures acetylcholinesterase activity in unwashed erythrocytes and butyrylcholinesterase activity in the plasma. The recommended substrate for both enzymes is 1.0 mM acetylthiocholine. The protocol is compared with other two recommended proto...

BACKGROUND Rhinophyma is an uncommon progressive disfiguring process of the lower nasal region that most commonly affects middle-aged white men. It is characterized by painless hyperplasia of the sebaceous and subcutaneous tissues, in the end stage, resulting in a bulbous nodular telangiectatic nose. Surgical modalities of treatment include electrosurgery, laser ablation, dermabrasion, cryosurg...