Introduction: IAChE (acetylcholinesterase) is one of the plasma’s enzymes that plays a crucial role in nervous signal transduction in synapses and is responsible for the hydrolysis of Ach. Material & Method: We investigated three methods for the determination of AChE in human blood serum, using three different assays. Michel methods are based on the measurement of hydrogen ion produced in pro...

OBJECTIVE To compare the efficacy and safety of four radiofrequency generators (Ellman, Select Sutter, Coblator, Somnus) for the treatment of simple snoring. MATERIALS AND METHODS Multicenter, randomized, prospective single-blind study on 120 selected patients with simple snoring (apnea/hypopnea index <10/h of sleep). Snoring sound intensity was measured on a visual analog scale and the partn...

BACKGROUND Rhinophyma is an uncommon progressive disfiguring process of the lower nasal region that most commonly affects middle-aged white men. It is characterized by painless hyperplasia of the sebaceous and subcutaneous tissues, in the end stage, resulting in a bulbous nodular telangiectatic nose. Surgical modalities of treatment include electrosurgery, laser ablation, dermabrasion, cryosurg...

This method is author’s innovative technique which follows number of applied surgical procedures on palate and uvula with only goal to advance functional results and avoid complications like velofaringeal insufficiency. Task of this method is to solve uvulopalato lateral obstruction and to expand oropharyngeal airway. It was introduced in the year 2000 by Dr. Vukoje. It requires strict indicati...

introduction: iache (acetylcholinesterase) is one of the plasma’s enzymes that plays a crucial role in nervous signal transduction in synapses and is responsible for the hydrolysis of ach. material & method: we investigated three methods for the determination of ache in human blood serum, using three different assays. michel methods are based on the measurement of hydrogen ion produced in proce...

Environmental Health Perspectives • volume 120 | number 2 | February 2012 A 55 Food Additives (JECFA), which considered but did not include naphthalene among geno toxic and carcino genic PAHs evaluated (FAO/WHO 2006). Although well intentioned, the LOC values suggested by Rotkin-Ellman et al. (2012) do not appear to take into account the natural background occurrence of PAHs in foods in many ca...

Organophosphorus compounds (OPs) are potent inhibitors of acetylcholinesterase (AChE). Treatment for OP poisoning is by administration of atropine sulfate, an oxime, and diazepam. Oximes such as 2-PAM are used to reactivate OP-inhibited AChE so as to restore normal enzymatic function and serve as a true antidote. There are reports of non-enzymatic hydrolysis by oximes of acetylthiocholine in in...

An electrochemical sensor is introduced as a tool applicable for diagnosis of intoxication by cholinesterase inhibitors caused by the well-known nerve agent VX. The traditional Ellman method was chosen for comparison with the sensor's analytical parameters. Both methods are based on estimation of blood cholinesterase inhibition as a marker of intoxication. While Ellman´s method provided a limit...

The procedure of Dietz et al. (Clin. Chem. 19: 1309-1313, 1973) for plasma cholinesterase (EC 3.1.1.7) gives a background absorbance of 1.4 A when extended to erythrocyte cholinesterase (EC 3.1.1.8) measurement, because the peak absorbance of the reaction product, 5-thionitrobenzoate, coincides with the hemoglobin Soret band at 410 nm. Consequently, the precision of erythrocyte cholinesterase m...