during the summer of 2004, a survey was conducted to determine the incidence and distribution of beet necrotic yellow vein virus (bnyvv) and its vector (polymyxa betae keskin) in marvdasht, fars province of iran.  a total of 353 root samples were collected randomly as well as 124 samples from plants showing rhizomania- associated symptoms.  the presence of bnyvv in the samples were tested by tr...

beet necrotic yellow vein benivirus (bnyvv) as one of the most important virues of sugar beet is the causal agent of rhizomania. bnyvv, as a type member of genus benyvirus has four types a, b, p and j, that are restricted to specific geographic areas. types a and b are similar in pathogenicity, but p and j types that have rna5 are more aggressive. these types cannot be distinguished serological...

Two murine monoclonal antibodies (MAbs) (2B7 and 46E9-9) reactive with the H7 flagellar antigen of Escherichia coli were produced and characterized. A total of 217 E. coli strains (48 O157:H7, 4 O157:NM, 23 O157:non-H7, 22 H7:non-O157, and 120 non-O157:nonH7), 17 Salmonella serovars, and 29 other gram-negative bacteria were used to evaluate the reactivities of the two MAbs by indirect enzyme-li...

The serological reactivity in indirect ELISA of five different bluetongue virus (BTV) serotypes (4, 10, 15, 16 & 20) was compared using polyclonal antisera raised against virus particles and an outer structural protein, VP2. Rabbit and sheep antisera against BTV-10 produced higher ELISA values with their homologous antigens than with heterologous serotypes. A hyperimmune rabbit serum specific f...

beet necrotic yellow vein virus (bnyvv) is the causal agent of sugar beet rhizomania, disease .  in the present study which was conducted in 2005 in shiraz, iran, serological methods (including elisa) were used for detection of disease and screening of disease tolerant germplasm in sugar beet breeding programs. for doing this, iranian isolate of bnyvv was purified and a specific antiserum was p...

The study recorded for the first time in Egypt presence of tomato spotted wilt virus (TSWV) on cucurbits, which was confirmed using several serological tests, like double antibody sandwich ELISA (DAS-ELISA); Dot-blot assay and Immuno capture polymerase chain reaction (IC-PCR).  By Dot blot immunoassay test against TSWV antisera, ability thrips species Thrips tabaci (Lind), palmi (Karny), Frankl...

Several enzyme-linked immunosorbent assays (ELISAs) for the detection of filovirus-specific antibodies have been developed. However, diagnostic methods to distinguish antibodies specific to the respective species of filoviruses, which provide the basis for serological classification, are not readily available. We established an ELISA using His-tagged secreted forms of the transmembrane glycopro...