in recent years, symptoms of virus diseases on stone fruits have been observed in wide range in golestan province. in order to study the important viruses on stone fruits, cherry, plum, nectarine, peach and apricot trees with symptoms such as mosaic, chlorosis, ring spot, shot hole, pale margins in leaves and dwarf in trees, sampling was done during 2005. putative infected plants were tested by...

Thermotherapy and chemotherapy in vitro were applied to eliminate Apple chlorotic leaf spot virus (ACLSV) and Prunus necrotic ring spot virus (PNRSV) from myrobalan (Prunus cerasifera var. divaricata Borgh.), PNRSV from ‘Empress’ plum (Prunus domestica L.) and Prune dwarf virus (PDV) from ‘Early Rivers’ sweet cherry (Cerasus avium (L.) Moench.) plants. ELISA assays conducted one year after ther...

Two methods (DAS-ELISA and RT-PCR) were compared for the detection of Prunus necrotic ringspot virus (PNRSV) in woody host Prunus mahleb isolated from Malatya. Total RNA extractions were made from serially diluted fresh leaf tissue, root, bark and one year old green bark tissue using silica-based method. Purified total RNA extracts were used as template for cDNA synthesis by reverse transcripti...

Amaç: Bu çalışmada, Adıyaman ili genelinde yetişen badem ağaçlarında apple mosaic virus (ApMV), prunus necrotic ring spot (PNRSV), prune dwarf (PDV), plum pox (PPV) ve chlorotic leaf (ACLSV)’lerinin Double Antibody Sandwich-Enzyme Linked Immuno Sorbent Assay (DAS-ELISA) Reverse Transcription-Polymerase Chain Reaction (RT-PCR) yöntemleriyle belirlenmesi, elde edilen virüs izolatlarının filogenet...

Sour cherry cv. Marasca is considered a native Croatian variety with the best fruit quality in Northern Dalmatia region. The current production not sufficient to cover demand, therefore new orchards should be established pathogen-tested planting material. To select virus- and bacteria-free mother trees, survey was conducted largest plantation "Vlačine". ELISA on 205 trees (51 elite, 103 average...

Prunus necrotic ringspot virus (PNRSV) causes yield loss in most cultivated stone fruits, including sweet cherry. Using a small RNA deep-sequencing approach combined with end-genome sequence cloning, we identified the complete genomes of all three PNRSV strands from PNRSV-infected sweet cherry trees and compared them with those of two previously reported isolates.

Sweet cherry (Prunus avium L.) is one of the most important stone fruit species in Bulgaria. The known to be susceptible more than 30 viruses. Among them are prune dwarf virus (PDV), prunus necrotic ringspot (PNRSV) and apple mosaic (ApMV), ilarviruses. Testing for these viruses required certification schemes order provide virus-tested propagated material fruit-growing industry prevent their di...

PCR amplicon next generation sequencing (NGS) analysis offers a broadly applicable and targeted approach to detect populations of both high- or low-frequency virus variants in one or more plant samples. In this study, amplicon NGS was used to explore the diversity of the tripartite genome virus, Prunus necrotic ringspot virus (PNRSV) from 53 PNRSV-infected trees using amplicons from conserved g...

RNA silencing is a powerful technology for molecular characterization of gene functions in plants. A commonly used approach to the induction of RNA silencing is through genetic transformation. A potent alternative is to use a modified viral vector for virus-induced gene silencing (VIGS) to degrade RNA molecules sharing similar nucleotide sequence. Unfortunately, genomic studies in many allogamo...