Suitable conditions for protein crystallization are commonly identified by screening combinations of independent factors that affect crystal formation. Because precipitating agents are prime determinants of crystallization, we investigated whether a systematic exploration of combinations of mechanistically distinct precipitants would enhance crystallization. A crystallization screen containing ...

Protein crystallization is frequently induced by the addition of various precipitants, which directly affect protein solubility. In addition to organic cosolvents and long-chain polymers, salts belong to the most widely used precipitants in protein crystallography. However, despite such widespread usage, their mode of action at the atomistic level is still largely unknown. Here, we perform exte...

BACKGROUND The aim of this study was to determine the prevalence and nature of self-perceived seizure precipitants in epilepsy patients, and evaluate whether anxiety level, depression, or health locus of control influence self-perception of seizure precipitants. METHODS Adults aged 18 and older who had epilepsy for at least 1 year were recruited in either the inpatient epilepsy monitoring uni...

We evaluated self-perception of seizure precipitants in 149 adult subjects with epilepsy: 71% of the subjects reported at least one factor that, according to the perception, increased the risk of suffering from a seizure. The subjects most often reported psychological stress, change of weather and sleep deprivation. Among the disease-related factors, seizure frequency and the state of conscious...

BACKGROUND Guidelines have proposed that atrial fibrillation (AF) can occur as an isolated event, particularly when precipitated by a secondary, or reversible, condition. However, knowledge of long-term AF outcomes after diagnosis during a secondary precipitant is limited. METHODS AND RESULTS In 1409 Framingham Heart Study participants with new-onset AF, we examined associations between first...

This study was designed to assess the effects of behavioural treatment on seizure recurrence, on seizure precipitants, and on associated psychiatric disturbances in 22 adolescents and young adults (age range 16-32 years, male : female ratio 10 : 12) with juvenile myoclonic epilepsy (JME) who showed seizure relapses despite rational AED therapy. The neurological assessment and a systematic searc...

the electrohydrodynamic (EHD) flow in a relatively wide electrostatic precipitator (ESP) (width:height = 2) is 2-or 3-dimensional (3D). The previous our measurements of 3D time-averaged flow velocity fields suggested that the flow in the ESP duct centre is almost 2-dimensional. In this paper we present both the 3D instantaneous and time-averaged flow velocity fields in a relatively wide ESP (wi...

Protein crystallization has been revolutionized by the introduction of high-throughput technologies, which have led to a speeding up of the process while simultaneously reducing the amount of protein sample necessary. Nonetheless, the chemistry dimension of protein crystallization has remained relatively undeveloped. Most crystallization screens are based on the same set of precipitants. To add...