The molecular epidemiology of nosocomial rotavirus infection in children admitted to the paediatric ward of a large hospital in southern India was studied by transmission electron microscopy and RNA electrophoresis on stool samples. Six hundred and twenty-six samples were collected and rotaviruses were detected in 35 of them. A predominant electropherotype was found in all the samples, and ther...

Rotaviruses are major cause of acute diarrhea in animals and humans which can result in huge economic losses in farm animals including pigs. We collected 195 samples of feces of diarrhoeic animals. Rotavirus was demonstrated by electron microscopy using the method of negative staining in 27 samples and by ELISA test using monoclonal antibodies to the group antigen VP6 in 44 samples. Nine sample...

The aim of our study was to determine whether the severity of rotavirus gastroenteritis may be related to the different characteristics of infecting viral strains. The severity of clinical symptoms in 401 children with acute rotavirus gastroenteritis was assessed using a scoring system for frequency and duration of vomiting, diarrhea, and fever, as well as the patients' requirements for intrave...

By enzyme-linked immunosorbent assay with group A-, subgroup-, and serotype-specific monoclonal antibodies (MAbs), we tested 414 stool specimens collected from pediatric and adult patients hospitalized with acute gastroenteritis between January and June 1988. Of 414 specimens tested, 124 (30%) were positive for group A rotavirus. The subgroup was determined in 110 specimens (88.7%); 16.1% were ...

Thirty-six of 37 rotavirus strains recovered from the diarrheal stools of 166 children <3 years of age in Burkina Faso were characterized at both the antigenic and molecular levels. The rotavirus strains were confirmed by polyacrylamide gel electrophoresis; 30 displayed predominantly short electropherotype patterns, and 6 had a long RNA pattern. The strains were subgrouped by monoclonal antibod...

The objective of this study was to ascertain the extent changes have occurred in the epidemiology of human rotavirus electropherotypes from the same location 7 to 8 years after an earlier study. Genomic RNA profiles of rotaviruses from diarrhoeic children admitted to the Kuala Lumpur Hospital from April to December 1996 were determined by polyacrylamide gel electrophoresis and silver staining. ...

introduction: in developing countries, rotaviral diarrhea is the cause of death of many children, and is the main etiology of gastroenteritis in children under 2 years old. determination of the prevalence of rotaviral infection can reveal the impact of this infection in this group of the population and provides clues as to the strategies for prevention and treatment protocols for children suffe...

Group A and group B rotaviruses are important diarrhea causing agents among calves and buffalo calves. Epidemiological studies in Indian calves revealed the predominance of group A rotavirus strains with G6, G8, and G10 specificity and group B rotaviruses. A total of 95 fecal samples were collected from calves and buffalo calves affected with diarrhea from an unorganized cattle farm and two cat...

Rotavirus A causes severe diarrhoea in infants and young children worldwide. The migration pattern (electropherotype) of the double-stranded RNA genome upon polyacrylamide gel electrophoresis has been used to define "strains" in molecular epidemiology. In temperate countries, distinct electropherotypes (strains) appear after the annual, off-seasonal interruption of rotavirus circulation. In Nep...