the aim of this study is to investigate antibacterial effects of immunodominant proteins isolated from the venom of naja naja oxiana snake against staphylococcus aureus, escherichia coli, bacillus subtilis and pseudomonas aeruginosa.the innate immune system is an important line of defense against bacterial diseases. antibacterial proteins produced by snake venoms have recently attracted signifi...

partially purified antigenic fractions of leishmania major promastigotes were obtained by sodium dodecyl sulfate (sds-page) and electro-elution. three isolated protein fractions designated as frac tions (fr.) 1, 2, and 3 correspond to 40-60, 60-80, 80-100 kilodalton (kda) respectively. they were used for immunization of balb/c mice against leishmaniasis. the effects of these fractions on immune...

Snake venom is a complex mixture of proteins, peptides, enzymes, carbohydrates, and minerals. They contain a variety of chemicals with pharmacological and toxicological properties. The innate immune system is the first line of defense against toxins and microbes. Antibacterial and anticancer proteins produced by snake venom have recently attracted significant attention due to their relevance to...

The protocol consists of running a native gel with in-gel digestion by proteases, subsequent mass spectrometrical determination of protein sequence and modifications, followed by electro-elution and conformational analysis using melting point and circular dichroism. Finally, the eluted protein is tested for preserved function. Herein, C1 esterase inhibitor is applied on a native gel; ingel dige...

Silver sulfadiazine is used to prevent and treat infections of second- and third-degree burns. It kills a wide variety of bacteria. In this study silver sulfadiazine was used in gelatin based electro-spun nano-fibers with various drug to polymer ratios (0, 5, 10, 15 and 20 %). SEM, EDX and FTIR analysis showed that the continuous, bead-free, fine fibers containing silver sulfadiazine as an anti...

silver sulfadiazine is used to prevent and treat infections of second- and third-degree burns. it kills a wide variety of bacteria. in this study silver sulfadiazine was used in gelatin based electro-spun nano-fibers with various drug to polymer ratios (0, 5, 10, 15 and 20 %). sem, edx and ftir analysis showed that the continuous, bead-free, fine fibers containing silver sulfadiazine as an anti...

Partially purified antigenic fractions of Leishmania major promastigotes were obtained by sodium dodecyl sulfate (SDS-PAGE) and electro-elution. Three isolated protein fractions designated as frac‌tions (Fr.) 1, 2, and 3 correspond to 40-60, 60-80, 80-100 kilodalton (kDa) respectively. They were used for immunization of BALB/c mice against Leishmaniasis. The effects of these fractions on immune...

A facile and efficient molecularly imprinted polymer (MIP) recognition element of electrochemical sensor was fabricated by directly electro-polymerizing monomer o-phenylenediamine (oPD) in the presence of template quinoxaline-2-carboxylic acid (QCA), based on one-step controllable electrochemical modification of poly(pyrrole)-graphene oxide-binuclear phthalocyanine cobalt (II) sulphonate (PPY-G...

the aim of this study was to assess the effect of bleaching on elution of monomers from nanofilled and microhybrid composites.80 samples (5mm diameter and 3mm thickness) of each composite were prepared. after curing, half of them were randomly polished. each group was divided into 8 subgroups and immersed in water or 10%, 20% and 30% h2o2 for 3 or 8 hours. eluted bis-gma (bis-phenol a glycidyl ...

The aim of this study is to investigate antibacterial effects of immunodominant proteins isolated from the venom of Naja Naja Oxiana snake against Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa.The innate immune system is an important line of defense against bacterial diseases. Antibacterial proteins produced by snake venoms have recently attracted signifi...