We have used the whole cell patch-clamp method to study pharmacological properties of low-voltage-activated (LVA) Ca2+ current in freshly dissociated neurons from dorsal root ganglia of adult rats. Inward barium current [in the presence of internal fluoride to reduce L-type high-voltage-activated (HVA) and external 1 microM omega-conotoxin GVIA to block N-type HVA current- was evoked from negat...

background: the entrance of toxic compounds into wastewater treatment plants (wwtps) can induce disorder in their biological units. therefore, it is necessary to determine the sources of toxic wastewaters. in this study, the toxicity of the wastewater of 3 metal plating industries was assessed using sequencing batch reactor (sbr) bacteria. methods: to obtain the 1-1000 ml/l concentrations, the ...

Haematoxylum campechianum is a medicinal plant employed as an astringent to purify the blood and to treat stomach problems such as diarrhea and dysentery. A bio-guided chemical fractionation of the methanolic extract obtained from this plant allowed for the isolation of five compounds: two chalcones known as sappanchalcone (1); 3-deoxysappanchalcone (2); three homoisoflavonoids known as hematox...

Results ADP (EC50: 7.5 μM), ATP (EC50: 0.5 μM), UTP (EC50: 0.8 μM), and thio-UTP (EC50: 0.4 μM) increased the number of action potentials fired in response to current injection; UDP failed to affect action potential firing. The effect of ADP was attenuated by a P2Y1 antagonist. This enhancement of excitability was abolished by flupirtine (30 μM), a KV7 channel opener, and slightly, but insignif...