Attaching and effacing Escherichia coli is a new causal agent of diarrhea in calves. Its major virulence factors are the intimin protein, encoded by the eaeA gene, and the Shiga-like toxins, encoded by slt genes. Because the sequences of these genes are available, we selected specific primers to amplify each virulence gene so as to develop a new identification test based on multiplex amplificat...

A promoterless gene for the Shiga toxin 1 B subunit (stxB1) has been placed under transcriptional control of the Vibrio cholerae heat shock gene htpG. A chromosomal enterohemorrhagic Escherichia coli fragment containing eaeA and 400 bp of upstream DNA was added to the construct, downstream of stxB1; no transcription terminators were located between the two genes. The plasmid construct was confi...

A total of 110 Escherichia coli strains of serogroup O119 were examined for the presence of virulence properties characteristic of enteropathogenic E. coli (EPEC). Three virulence patterns were distinguished based on the detection of a chromosomal gene mediating intimate attachment (eaeA) and plasmid DNA involved in localized adherence (EAF and bfpA). The first pattern, represented by strains w...

the aim of this study was to isolate escherichia coli from chickens and to determine the presence of the eaea gene, a virulence factor detected in escherichia coli, in the isolates by polymerase chain reaction (pcr). different chicken organs (lung, liver and spleen) were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. escherichiacoliwas isolated from ...

BACKGROUND & OBJECTIVES Limited information is available on shiga toxin producing Escherichia coli (STEC) in animals and birds from India. An outbreak of acute diarrhoea in poultry birds at Aizawl, Mizoram was investigated for detection and characterization of STEC and enteropathogenic E. coli (EPEC). METHODS E. coli was isolated and identified from rectal swabs, intestinal contents, heart bl...

BACKGROUND AND OBJECTIVES Shiga toxin-producing Escherichia coli (STEC) have emerged as human pathogens and contamination of foods of animal origin has been a major public health concern. The aim of the present study was to determine the dissemination of STEC in healthy and diarrheic calves in Urmia region which is located in West Azerbaijan province, Iran. MATERIALS AND METHODS In the curren...

The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in Escherichia coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs (lung, liver and spleen) were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. Escherichiacoliwas isolated from ...

Enteropathogenic Escherichia coli (EPEC) infections are a leading cause of infant diarrhea in developing countries. Recently eaeA, a gene necessary for the characteristic intimate attachment of EPEC to epithelial cells in tissue culture, was described. We conducted a randomized, double-blind study to determine the role of the eaeA gene in human EPEC infection. 11 adult volunteers ingested 2 X 1...

The aim of this study was to identify virulent Shiga toxin-producing Escherichia coli (STEC) strains isolated from faecal samples of 100 clinically healthy calves. In the present study, a total of 100 Escherichia coli (E. coli) isolates from clinically healthy calves belonging to 6 different farms located in Khorasan Razavi province, Iran, were examined for presence of virulence genes character...

The present work was conducted to compare the occurrence of Escherichia coli possessing virulence and ESBL genes in backyard and farmed poultry. Three hundred and sixty samples from the poultry kept in backyard system and 120 samples from the farmed birds were collected from West Bengal, India. Among the E. coli isolates of backyard poultry (O2, O10, O25, O55, O60, O106, UT), none of them posse...