Abstract Three different dosimetry systems were prepared based on diphenyl thiocarbazone (DTH) dye: polymer films made with, poly(vinyl alcohol), DTH liquid dosimeter and gel dosimeter. All the received several gamma doses. The three analyzing by UV–Vis spectrophotometery before after irradiation. effective dose range in linear part of these is between 1–10 kGy, 0.3–2 50–200 Gy. dosimeters reli...

Using peripheral blood mononuclear cells (PBMC) from individuals with or without coccidioidal delayed-type hypersensitivity (DTH), we examined and attempted to modulate the in vitro responses of PBMC from various donors to the coccidioidal antigen toluene spherule lysate (TSL). Among healthy DTH-positive donors, 100 ng of human recombinant interleukin-10 (IL-10) per ml suppressed both PBMC prol...

Effect of Neurotropin on the delayed type hypersensitivity (DTH) response to sheep red blood cells was examined in mice. Neurotropin suppressed the high DTH responses in ddY and BALB/c mice, while it enhanced the low DTH response in C57BL/6 mice when administered after sensitization. In addition, Neurotropin exerted a suppressive effect on the enhanced DTH response by cyclophosphamide pretreatm...

The contribution that antibody and delayed type hypersensitivity (DTH) make in promoting HSV-1 clearance from the infected cornea was investigated. Balb/c mice were immunized intravenously or subcutaneously with an attenuated strain of HSV-1 to generate hosts which were antibody-producing DTH-tolerant or antibody-producing DTH-responsive. Anti-mu serum treated mice were likewise sensitized intr...

Delayed-type hypersensitivity (DTH) to phosphorylcholine (PC) could be elicited by mixtures of monoclonal anti-idiotypic antibodies. Using this system in DTH transfers, the question was asked of whether anti-idiotypic antibodies could elicit antigen induced DTH in H-2-incompatible mice. Transfer of PC-activated BALB/c lymph node cells into BALB.K mice and subsequent elicitation with anti-idioty...

IL-18 (formerly known as IFN-gamma-inducing factor) enhances Th1 responses via effects that are thought to be dependent on and synergistic with IL-12. The potential for IL-18 to exert IL-12-independent effects in delayed-type hypersensitivity (DTH) responses was studied in a model of Th1-directed, DTH-mediated crescentic glomerulonephritis induced by planting an Ag in glomeruli of sensitized mi...

PURPOSE To investigate the role of the eye and the spleen in maintaining suppression of delayed-type hypersensitivity (DTH) after anterior chamber (AC) inoculation of allogeneic splenocytes. METHODS Suppression of DTH response was tested in BALB/c mice after AC inoculation of allogeneic B10.D2 splenocytes. Seven days after AC injection, the antigen-inoculated eyes were enucleated or the splee...

The effect of oral administration of a polysaccharide (KGF-C), isolated from the kefir grain, on delayed-type hypersensitivity (DTH) induced by picryl chloride and on the growth of solid tumor was examined in mice. KGF-C caused an increase in DTH response in intact mice and also tumor-bearing mice. The growth of tumor inoculated after the DTH test was markedly inhibited in the groups with high ...

Purified protein derivative (PPD) is a widely used reagent for the diagnosis of Mycobacterium tuberculosis infection. Recently, the molecular composition of PPD was defined, with hundreds of mycobacterial protein representatives making up PPD. Which, if any, of these specific products drive the potency of PPD remains in question. In this study, two proteins (DnaK and GroEL2) previously identifi...

Leishmania tropica and L. major are etiologic agents of human cutaneous leishmaniasis. Delayed type hypersensitivity (DTH) is an immunologic response that has been frequently used as a correlate for protection against or sensitization to leishmania antigen. In BALB/c mice, L. tropica infection results in non-ulcerating disease, whereas L. major infection results in destructive lesions. In order...