low efficiency of callus induction and green-plant regeneration have limited the application of anther culture in breeding programs. this study was conducted to determine the inheritance of callus induction and plant regeneration in rice (oryza sativa) anther culture. ten genotypes drived from a five-parent diallel mating set contain khazar, sepidrood, hashemi, alikazemi and nematt were evaluat...

abstract  in order to study f3´5´h and f3´h function and their intraction in the grapevine flavonoids biosynthetic pathway, designing and constraction of suitable constructs encoding ihprna was performed and transgenic lines containing silencing construct of f3´h and simultaneous silencing construct of f3´5´h and f3´h were created. for this purpose, fragments were selected from both genes and i...

Successful anther/microspore culture largely depends on the use of microspores at appropriate developmental stages at the time of culture, which can be specific for each plant species and genotype. This study was carried out to determine the correlation between morphological characteristics, namely floral bud (FB) length and width and anther length, and specific microspore developmental stages ...

Anther cultures have been developed from transgenic donor peppers carrying HPT/J1-1. Eight out of sixteen plants produced from an anther culture set pepper fruits. Southern blot analysis of donors revealed two independent plants with a single copy of the integrated transgene. PCR and RT-PCR results showed the inheritance of HPT/J1-1 and expression of J1-1 in A1. All A1 progeny derived from tran...

Pollen plants from anther culture in rice breeding allows selection of fine strains in early generations. Exploitation of anther culture technique in research is limited due to very low regeneration frequency of indica cultivars and the transfer of anther culture traits from japonica to indica varieties is important in improving these rice varieties. In this study the optimization of media requ...

the objective of this study was to estimate genetic parameters and to investigate the type of gene action in controlling androgenesis in wheat. two wheat cultivars of grebe and houtman were reciprocally crossed with two synthetic genotypes of do1 and pol and then a complete set of the parents, f1, reciprocal f1 (rf1), f2 and back-cross generations (bc1 and bc2) of each cross were used for anthe...

this study involves in vitro androgenesis of zea maysl. via anther culture. combination of two embryoinduction media (imss & ypm) in presence of different colchicine concentrations (0, 100, 200, 250, 300and 400 mg/l ) in the pretreatment medium (iml) andpretreatment duration (0, 3, 6 and 9 days) in twogenotypes (dh5×dh7 and eth-m82) were tested.after colchicine pretreatment, anthers were tr...

The objective of this study was to estimate genetic parameters and to investigate the type of gene action in controlling androgenesis in wheat. Two wheat cultivars of Grebe and Houtman were reciprocally crossed with two synthetic genotypes of Do1 and Pol and then a complete set of the parents, F1, reciprocal F1 (RF1), F2 and back-cross generations (BC1 and BC2) of each cross were used for anthe...