نتایج جستجو برای: dot immunogold filtration assay

تعداد نتایج: 287086  

Journal: :iranian journal of parasitology 0
somayeh kordafshari dept. of parasitology, school of veterinary medicine, tehran university, tehran, iran. seyed hossein hosseini dept. of parasitology, school of veterinary medicine, tehran university, tehran, iran. fatemeh jalousian dept. of parasitology, school of veterinary medicine, tehran university, tehran, iran. masoumeh rajabibazl dept. of clinical biochemistry, school of medicine, shahid beheshti university, tehran, iran. malcolm jones dept. of parasitology, school of veterinary sciences, queensland university, queensland, australia. fazeleh etebar dept. of parasitology, school of veterinary medicine, tehran university, tehran, iran.

background: cystic echinococcosis is an important zoonosis caused by the echinococcus granulosus with a substantial impact in human and animal health in en-demic areas. the purpose of the present study was serodiagnosis optimizing of dog echinococcosis in order to achieve a rapid diagnostic method. methods: eight dogs were challenged with protoscoleces in order to have posi-tive echinococcosis ...

2006
Liu Yisheng Du Wenping Zheng Kuiyang Fu Linlin Chen Ming

A fast, specific, sensitive, convenient, and economical rapid-dot-immunogold staining (R-Dot-IGS) assay was used to detect serum antibodies in patients infected with Schistosoma japonicum. The soluble egg antigen of Schistosoma japonicum was added onto microspore membrane. After pre-reacting and blocking, the serum to be detected and sheep anti-human IgG labeled with chloroauric acid were added...

2015
Somayeh KORDAFSHARI Seyed Hossein HOSSEINI Fatemeh JALOUSIAN Masoumeh RAJABIBAZL Malcolm JONES Fazeleh ETEBAR

BACKGROUND Cystic echinococcosis is an important zoonosis caused by the Echinococcus granulosus with a substantial impact in human and animal health in endemic areas. The purpose of the present study was serodiagnosis optimizing of dog echinococcosis in order to achieve a rapid diagnostic method. METHODS Eight dogs were challenged with protoscoleces in order to have positive echinococcosis se...

2004
Paula Célia Mariko Koga Caroline Anunciação Menezes Flávia Afonso Lima Júlia Mitico Nara Caroline Arantes Magalhães Aurora Marques Cianciarullo Luiz Rachid Trabulsi Roxane Maria Fontes Piazza

Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry and immunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizes the different intimin subtypes and suggests that it can be used as a tool for EP...

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